Structure of the human prohibitin complex in the closed state_EMDB-70179
By onStructure of the human prohibitin complex in the closed state
Structure of the human prohibitin complex in the open state
By onStructure of the human prohibitin complex in the open state
Zenodo_In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment
By onZenodo deposition for In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment
In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment_EMPIAR-12871
By onDataset of Oligomycin / Antimycin A treated mitochondria from U2OS cells
Transient Transfection of Cultured Mammalian Cells for Live 3D Fluorescence Microscopy
By onTransient Transfection of Cultured Mammalian Cells for Live 3D Fluorescence Microscopy
OA treatment and Quantification of Parkin recruitment to mitochondria
By onOA treatment and Quantification of Parkin recruitment to mitochondria
Live-cell Imaging of Mitochondrial Membrane Proteins in Cultured Mammalian Cells by Airyscan Laser Scanning Confocal microscopy
By onThis is a protocol for live-imaging of cultured mammalian cells transfected with plasmids encoding fluorescently-tagged mitochondrial proteins.
In situ Cryo ET data collection and processing with Scipion
By oncryo et data collection and subtomogram averaging using Scipion
pLV-BFP-Mito
By onLentivirus plasmid for the stable expression of mitochondrial matrix-localized BFP in mammalian cells
pLV-mCherry-Parkin
By onLentivirus plasmid for the stable expression of mCherry-Parkin in mammalian cells
EVscope: A Comprehensive Bioinformatics Pipeline for Accurate and Robust Analysis of 1 Total RNA Sequencing from Extracellular Vesicles
By onEVscope is a bioinformatics pipeline for processing EV RNA-seq datasets, improving read assignment with an EM algorithm. It generates BigWig files, corrects gDNA contamination, and infers RNA origins. The pipeline includes 19 RNA biotypes.
Autophagic stress activates distinct compensatory secretory pathways in neurons
By onNeurodegenerative diseases are linked to autophagy dysfunction causing harmful build-up of cellular waste. Neurons with Parkinson's mutation increase release of autophagic vesicles and exosomes, suggesting a protective response to chronic stress.
Apoptosis Detection with CellEvent Caspase-3/7
By onApproach combines CellEvent Caspase 3/7 reporter with Cellpose machine learning detection. Expanded methods applying these two approaches individually are available (see Invitrogen directions and Stinger and Pachitariu 2025).
Autophagic stress activates distinct compensatory secretory pathways in neurons data
By onPrimary data, raw images, and full western blots pertaining to "Autophagic stress activates distinct compensatory secretory pathways in neurons data" Palumbos et al., 2025.
Protein Extraction, Mass Spectrometry, and Data Analysis
By onProtocol describing protocol of protein extraction, mass spectrometry, and subsequent data analysis for Palumbos et al., 2025. All steps of the protocol were performed by CHOP PENN Proteomics core.
CalceinAM labeling of Extracellular Vesicles
By onProtocol used to visualize intact vesicles isolated from primary murine neurons. Protocol used in Palumbos et al., 2025 and adapted from https://doi.org/10.1371/journal.pone.0317689
Palumbos_LRRK2G2019S_CTRL_Secretome_smRNA_Transcriptomics
By onSmall RNA transcriptomic data profiling secretome of control and LRRK2(G2019S) murine cortical neurons.
