ASAP Collaborative Research Network (CRN) Impact Report 2025-2026
As an extension of the ASAP CRN Impact Report 2020–2024, this report highlights the cross-cutting scientific themes, field-enabling resources, infrastructure efforts, and key discoveries accelerating breakthroughs in PD research across our Collaborative Research Network (CRN).
Datasets for Belal et al. ‘Cholinergic interneuron control of GABAergic circuits targeting spiny projection neurons is disrupted in parkinsonian models’
By onDeposit contains processed data spreadsheets and SVG figure panels generated from the raw electrophysiology, imaging and in-situ hybridization recordings.
Culturing, transfection and NBD-labeled lipid uptake assay in HeLa-CDC50A-OE cells
By onAssay for NBD-labeled lipid uptake in HeLa-CDC50A-OE cells includes seeding, transfection, and flow cytometry to measure flippase construct activity, enabling quantitative assessment of lipid uptake.
Flow cytometry data from the manuscript “Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mamma-lian Cells”
By onSummary: The manuscript presents a fluorescence-gated flow cytometry method to measure lipid flippase activity in mammalian cells.
Single-nucleus RNA (snRNA) sequencing
By onStep-by-step protocol describing the snRNA sequencing workflow for nuclei isolated from post-mortem human brain tissue.
Image resources (Garza et al. 2026)
By onBrightfield, Immunofluorescent images of KOLF2.1-derived microglia (MGlia) and RC17 hES cells derived dopaminergic neurons by Garza et al (2026).
Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency
By onPathogenic variants in ATP13A2 cause Kufor-Rakeb syndrome & early-onset parkinsonism. Spermidine supplementation rescues symptoms by counteracting microglia dysfunction & improving neuronal integrity.
iPSC QC (Garza et al, 2026)
By onQuality control reporting table and imaging files for hiPSCs and hESCs lines used in Garza et al., 2026.
Mathematica code for Astrocytes mediate the dopaminergic modulation of tonic GABAergic signaling in substantia nigra
By onMathematica notebook containing functions used to measure spikerates, draw histograms, and extract current traces from Clampfit raw data exports.
Dandiet: Astrocytes mediate the dopaminergic modulation of tonic GABAergic signaling in Substanta nigra
By onThis is a Dandiet that hosts binary files of electrophysiology data for the titled manuscipt 'Astrocytes mediate the dopaminergic modulation of tonic GABAergic signaling in Substanta nigra'.
Fushiki et al – Video Recording of Parkinson’s Disease Model Mice
By onRaw behavioral datasets and experimental video recordings referenced in the ).
Imaging Dataset for: A Vulnerable Subtype of Dopaminergic Neurons Drives Early Motor Deficits in Parkinson’s Disease
By onDataset includes imaging data from experiments detailed in PMID: 39763754. Coronal brain sections imaged with fluorescence microscopy to assess reporter and transgene expression.
EMG fabrication in Rodrigues-Vaz & Athalye et al
By onThis is the protocol for fabrication of EMG electrodes for bulk muscle recordings used for Rodrigues-Vaz and Athalye et al, 2025 and based on Akay et al, 2006.
Histology from Rodrigues-Vaz and Athalye et al: Imaging of specific mouse brain region where viral injection and implant was placed at
By onThis dataset is part of an experiment in which dorsolateral striatum (DLS) was imaged while mice performed task. This images show targeting of viral expression and lens implant to DLS.
Virus Injections from Rodrigues-Vaz & Athalye et al
By onThis is the standard stereotaxic/Nanoject injection mouse surgery protocol for Rodrigues-Vaz and Athalye et al, 2025.
Code used to analyze striatum population dynamics during isometric forelimb actions
By onCode used for analysis and figures for paper titled: "Striatal ensembles specify and control granular forelimb actions".
2-photon imaging raw data from striatal populations through GRIN lens
By onThe neuronal activity of striatal cells was acquired while the mouse performed a two-action isometric forelimb force task.
Holographic stimulation of neuronal ensembles in striatum
By onThis dataset includes all the raw data regarding the experiments with 2-photon imaging, isometric force task and holographic stimulation in Rodrigues-Vaz & Athalye et al.
NERINE reveals rare variant associations in gene networks across phenotypes and implicates an SNCA-PRL-LRRK2 subnetwork in Parkinson’s disease
By onNERINE is a rare variant association test that links rare genetic variation with gene network topology to improve discovery power in complex diseases. NERINE implicates an α-synuclein/prolactin stress-response in Parkinson’s disease.
all protocols related to “Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency”
By onAll protocols related to "Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency"
