Adoptive transfer of mitochondrial antigen-specific CD8+ T-cells in mice causes parkinsonism and compromises the dopamine system
By Emma Sherrell onThe degeneration of dopamine neurons in the ventral midbrain is linked to the development of motor symptoms in Parkinson's disease (PD). Evidence suggests that neuroinflammation and mitochondrial dysfunction drive neurodegenerative mechanisms in PD. Results provide evidence for mitochondrial-specific CD8+ T cell infiltration in the brain in driving PD-like pathology.
LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3
By Emma Sherrell onThe Leucine-rich repeat kinase-2 (LRRK2) G2019S mutation is one of the well-recognized genetic risk factors in Parkinson's disease (PD). Increased LRRK2 activity was also observed in immune cells from PD patients. The authors generated and characterized a new T cell receptor (TCR) transgenic mouse strain bearing LRRK2 G2019S knock-in mutation.
Deep sequencing of proteotoxicity modifier genes uncovers a Presenilin-2/beta-amyloid-actin genetic risk module shared among alpha-synucleinopathies
By Emma Sherrell onConventional genetic analyses are underpowered to address whether neurodegenerative diseases linked to misfolding of the same protein share genetic risk drivers or whether different protein-aggregation pathologies in neurodegeneration are mechanistically related. The authors study patients based on protein aggregation phenotype to detect variants in a targeted set of genes.
Alpha-synuclein aggregates trigger anti-viral immune pathways and RNA editing in human astrocytes
By Emma Sherrell onParkinson's disease is a neurodegenerative disease characterized by a proteinopathy with marked astrogliosis. To investigate how a proteinopathy may induce a reactive astrocyte state, and the consequence of reactive astrocytic states on neurons, the authors generated hiPSC-derived astrocytes, neurons, and co-cultures and exposed them to small soluble alpha-synuclein aggregates.
Dopamine neuron activity encodes the length of upcoming contralateral movement sequences
By Emma Sherrell onThe relationship between the activity of dopaminergic neurons (DANs) and the length of movement sequences is unknown. The authors imaged the activity of SNc DANs in mice. Results indicate that movement-initiation DANs encode more than a general motivation signal and invigorate aspects of contralateral movements. View original preprint.
His-ATG3
By Emma Sherrell onPlasmid: ATG3 protein (WT) overexpression in E.Coli. 6His-TEVsite-ATG3 WT-stop.
His-ATG3-C264A
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stop.
His-ATG3-Y209A
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y209A-stop.
His-ATG3-Y210A
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y210A-stop.
His-ATG3-T244A
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-T244A-stop.
His-ATG3-H262A
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H262A-stop.
His-ATG3-R265A
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop.
His-ATG3-H266A
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop.
His-ATG3-K208D
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stop.
His-ATG3-K62D/K64D
By Emma Sherrell onPlasmid: ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop.
Oncogenic BRAF V600E induces glial proliferation through ERK and neuronal death through JNK
By Emma Sherrell onActivating V600E in BRAF is a common driver mutation in cancers of multiple tissue origins. BRAF V600E has also been implicated in neurodegeneration. The present study aims to characterize BRAF V600E on cell death and survival in three major cell types of the CNS: neurons, astrocytes, and microglia.
T cell differentiation from mice spleen tissue
By Emma Sherrell onThis protocol is for T cell differentiation from mice spleen tissue to investigate T cell function (including differentiation and proliferation) in vitro.
Genetic modification of human induced pluripotent stem cells (hiPSCs) by lentiviral transduction protocol
By Emma Sherrell onThis is the protocol for the genetic modification of human induced pluripotent stem cells (hiPSCs) using lentiviral transduction method.
Cytotoxicity Assay Protocol
By Emma Sherrell onThis is the protocol for cytotoxicity assay in cancer cell lines using flow cytometry.
Preparation of Holding ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By Emma Sherrell onHolding ACSF is used for incubating brain slices before cell-attached or whole-cell electrophysiological recordings of neurons.