Cell culture, transfection, immunocytochemistry, and imaging (iPSC, iNeuorn, DA neuron, RPE cells)
By onThis protocol describes the maintenance, transfection, immunocytochemistry, and imaging of RPE1 and also transfection, immunocytochemistry, and imaging of iPSCs, i3 Neurons and DA neurons.
Generation of knockout iPSCs using CRISPR-Cas9 genome editing
By onThis protocol describes the genetic modification of induced pluripotent cells (iPSCs) using CRISPR-Cas9, including synthesis of gRNA plasmids, transfection, selection of clones, and sequencing of genomic DNA to confirm knockout generation. The steps
Crude Subcellular fractionation of FAM177A1-GFP expressing cells
By onThis protocol details the crude subcellular fractionation of FAM177A1-GFP expressing cells.
Immunoblotting of i3 Neurons and dopaminergic neurons
By onThis protocol describes the preparation of cell lysate from and iPSC-derived neurons (I3 Neurons, dopaminergic) and the immunoblotting procedure.
Protocol for Neuronal Live-imaging of primary cultures
By onThis protocol introduces a concise method for live-imaging neuronal activity.
Protocols: Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits
By onThe current collection contains all experimental protocols associated with the study "Phosphoglycerate kinase is a central leverage point in Parkinson’s Disease driven neuronal metabolic deficits".
Primary hippocampal and cortical neuronal culture and transfection
By onThis protocol presents an established method to isolate and culture hippocampal and cortical neurons from rat and mouse brains along with a method to sparsely transfect these neurons.
Fluorescence size exclusion chromatography (FSEC) of native nanodiscs
By onThis is a protocol describing how to conduct Fluorescence size exclusion chromatography of native nanodiscs.
Expression and purification of Syp-VAMP2 complex into native nanodiscs
By onThis is a protocol for the purification of Syp-VAMP2 complex into native nanodiscs.
Step-centrifugation Assay
By onThis protocol details the protocol for conducting a step-centrifugation assay to determine the minimum required speed for full separation of vesicles from native nanodiscs.
Transmission Electron Microscopy of Native Nanodiscs
By onThis is a protocol for conducting transmission electron microscopy of native nanodiscs to determine population size distribution and morphology.
Native Nanobleach of native nanodiscs
By onThis is a protocol to conduct Native Nanobleach of native nanodiscs.
Database-driven protein solubilization
By onThis is a protocol for using the extraction database found at www.polymerscreen.yale.edu for optimal extraction of membrane proteins into native nanodiscs.
LC-MS of Native Nanodiscs
By onThis protocol provides a step-by-step guide for conducting LC-MS analysis of native nanodiscs extracted from biological membranes.
CHCHD2 mutant mice display mitochondrial protein accumulation and disrupted energy metabolism
By onMutations in mitochondrial protein CHCHD2 lead to a form of Parkinson's disease. CHCHD2 T61I mutant mice show molecular changes in the brain, altered metabolism favoring glycolysis, and disrupted mitochondrial function in dopamine neurons.
Spatial transcriptomics reveals molecular dysfunction associated with cortical Lewy pathology Dataset: Lewy pathology induced molecular dysfunction
By onSpatial transcriptomics data repository for Goralski et al., 2024 study on cortical Lewy pathology molecular dysfunction. Supplementary to computational notebook at DOI: 10.5281/zenodo.10732492.
Stereotaxic Injections- Mouse
By onThis protocol details mouse stereotaxic injections for any purpose, but in this case is primarily used for the injection of misfolded proteins.
Spatial transcriptomics reveals molecular dysfunction associated with cortical Lewy pathology Code: Goralsth/Spatial-transcriptomicSpatial transcriptomics Code: Spatial-transcriptomics-reveals-molecular-dysfunction-associated-with-cortical-Lewy-pathology
By onCode repository for Goralski, et., al., 2024.
Spatial transcriptomics reveals molecular dysfunction associated with cortical Lewy pathology Dataset: raw sequencing files
By onSpatial Transcriptomics data in pathology bearing neurons of PFF injected mice and Parkinson's disease human post mortem brain.
