Designing sgRNA Oligos and Inserting Guides into the GEARBOCS Vector
By onProtocol for designing sgRNAs for genes using GEARBOCS vector backbone enabling astrocyte-specific genetic manipulation in CRISPR/Cas9 mice for in vivo assays.
Baseline α-synuclein seeding activity and disease progression in sporadic and genetic Parkinson’s disease in the PPMI cohort
By onStudy analyzed α-synuclein seeding activity in Parkinson's patients from PPMI cohort. No statistically significant association found between baseline α-syn seeding activity and disease progression in sporadic PD, LRRK2 PD, or GBA PD.
DAB Immunohistochemistry (IHC) Staining for Stereological Analysis
By onThis protocol details the DAB immunohistochemistry staining for stereological analysis of 40 µM slices cut with cryostat and stored in antifreeze.
Behavioral analyses in mice
By onThis collection of protocols describes different behavioural assays performed in mice including, grip strength test, pole test, rotarod, open field and nose poking learning task
Perfusion/ Fixation for cryostat slicing for HHC
By onThis protocol details the Perfusion and Fixation for cryostat slicing for HHC.
Immunohistochemistry of free floating slices
By onThis protocol details the immunohistochemistry of free floating slices.
Assembloid model to study loop circuits of the human nervous system
By onHuman stem cells are used to create in vitro cortico-striatal-thalamic-cortical circuits, mimicking neural networks involved in neuropsychiatric disorders. This system allows study of normal development and diseases like autism and Tourette syndrome.
DIO-SPOTlight Transgenic Mouse to Functionally Monitor Protein Synthesis Regulated by the Integrated Stress Response
By onA new SPOTlight reporter allows visualization of the integrated stress response (ISR) activity in specific cell types in transgenic mice, aiding in studying health and disease implications.
Mitochondrial fission controls astrocyte morphogenesis and organization in the cortex
By onMitochondrial fission in astrocytes plays a key role in brain development. Loss of Drp1 disrupts mitochondrial distribution, leading to astrocyte morphological changes and organization disruptions, impacting neurodevelopment and Connexin 43 levels.
Protocol collection associated with “Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons”
By onThe protocol collection provides a toolkit for profiling endolysosomes in human induced neurons and hESCs using Endo-IP and Lyso-IP techniques.
Protocol collection for “Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST”
By onProtocol collection for "Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST"
Raw data associated with DOI: 10.7554/eLife.67900 (Pathogenic LRRK2 control of primary cilia and Hedgehog signaling in neurons and astrocytes of mouse brain)
By onImmunofluorescence microscopy images, raw immunoblotting data and tabular data used to generate graphs shown in all figures.
Collection of protocols for “PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection”
By onThis a collection of protocols for "PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection".
Bridge-like lipid transfer protein 3A (BLTP3A) is associated with membranes of the late endocytic pathway and is an effector of CASM
By onThe bridge-like lipid transfer protein, BLTP3A/UHRF1BP1, anchors VAMP7-positive vesicles to lysosomes and acts as an effector of CASM potentially to aid in the repair or mitigation of lysosomal damage.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder
By onA study identified novel lysosomal proteins in brain cell types, including SLC45A1 causing neurological disease by affecting lysosomal function and iron homeostasis. This work enhances understanding of lysosome biology in neurodegeneration.
RAW 264.7 LRRK2 KO + HALO-human LRRK2 (LIR1 mutant) + HA-human GABARAP
By onRAW 264.7 LRRK2 KO + HALO-human LRRK2 (LIR1 mutant) + HA-human GABARAP cell line
RAW 264.7 LRRK2 KO + HALO-human LRRK2 (LIR2 mutant) + HA-human GABARAP
By onRAW 264.7 LRRK2 KO + HALO-human LRRK2 (LIR2 mutant) + HA-human GABARAP cell line
RAW 264.7 LRRK2 KO + HALO-human LRRK2 (LIR1+2 mutant) + HA-human GABARAP
By onRAW 264.7 LRRK2 KO + HALO-human LRRK2 (LIR1+2 mutant) + HA-human GABARAP cell line
Spatial genomics of AAVs reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo
By onThe authors identify widespread transcriptional crosstalk between enhancers and promoters delivered in different AAV vectors. They show that this arises from concatemerization and harness it to deliver large cargo for cell type-specific gene editing.
Confocal imaging of transcriptional crosstalk between AAV genomes carrying different enhancers in the mouse brain
By onA data collection of images of brains of mice transduced with viral vectors carrying different regulatory elements and reporters to study transcriptional crosstalk between AAV genomes
