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Data and code for Active Escape (“Integrated Representations of Threat and Control in the Lateral Frontal Pole”)
onData, Code, and Key Resource Table used in the preprint from Stasiak et al., 2025 ("Integrated Representations of Threat and Control in the Lateral Frontal Pole").
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Active Escape Protocol (Stasiak et al., 2026)
onProtocol for Active Escape task by Stasiak et al., referenced in "Integrated Representations of Threat and Control in the Lateral Frontal Pole" (2026) manuscript.
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pCHMWS-hATP13A4_A356V-IRES-Puro
ontransfer plasmid for lentiviral vector production expressing Hs ATP13A4 A356V mutant
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pCHMWS-hATP13A4_A356V-Twinstrep-Flag-IRES-Puro
ontransfer plasmid for lentiviral vector production expressing Hs ATP13A4 A356V mutant with Twinstrep-Flag tag
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AAV-gfaABC1D-Lamp1-TurboID-HA
onUsing the gfaABC1D promoter expressing Lamp1-TurboID fusion protein
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pCHMWS-FLUC-IRES-Puro
ontransfer plasmid for lentiviral vector production expressing firefly luciferase (FLUC)
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AAV-gfaABC1D-cytoTurboID-HA
onUsing the gfaABC1D promoter expressing cytoplasmic TurboID with C-terminal HA tag
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Postnatal astrocyte labeling by electroporation
onHere, we provide a step-by-step guide to perform Postnatal Astrocyte Labeling by Electroporation (PALE), detailing the preparation, electroporation procedure, and post-electroporation analysis.
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in vivo TurboID
onProtocol uses iBioID with Lamp1-TurboID in astrocytes to study endo-/lysosomal protein interactions in mice. AAV delivery allows selective biotinylation for proteome mapping, offering insights into astrocyte signaling in vivo.
