A computational pipeline to quantify primary cilia in mouse embryonic fibroblasts with CellProfiler
Output Details
Description
We present here an automated CellProfiler (Stirling et al., 2021) software pipeline to quantify the number of primary cilia in cultured cells. The primary cilia were labeled using anti-Arl13b antibodies and nuclei were labeled using DAPI. This protocol works with .czi format images which are acquired using a Zeiss laser scanning confocal microscope and are maximum intensity projected.
Identifier (DOI)
10.17504/protocols.io.bp2l6x2j5lqe/v1
