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Acute Extracellular Multi-unit Recordings and Optotagging
Output Details
Description
This protocol details acute extracellular multi-unit recordings and optotagging in mice. Preparation involves setting up recording systems and optogenetic equipment. The mouse is anesthetized, placed in a stereotaxic frame, and subjected to a craniotomy for electrode placement. A multielectrode probe, combined with an optic fiber, is inserted into the target brain area. Optotagging involves light stimulation, monitored for effectiveness. Post-recording, the probe is removed and cleaned, and the mouse receives careful post-operative care to ensure recovery.
Identifier (DOI)
10.17504/protocols.io.14egn3qd6l5d/v1