Cell lysis and immunoblotting for protein and phospho-protein quantification

By Dan Dou, Alexander Boecker, MD and Erika Holzbaur, PhD
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Output Details

Description
Here, we describe the procedure by which human iPSC-derived neurons or mouse embryonic fibroblasts (MEFs) were lysed and probed for levels of proteins of interest using Western blot.
Identifier (DOI)
10.17504/protocols.io.5jyl8j5zrg2w/v1
Tags
  • Human
  • Mouse
  • Protein
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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