CRISPR/Cas9-Based Functional Genomics in Human Induced Pluripotent Stem Cell–Derived Models: Can “the Stars Align” for Neurodegenerative Diseases?

By Yu Chen, PhD, Chase Chen, MSc, Ellen Sidransky, MD and Tiffany Jong, BSc
View Published Article

Output Details

Description
Viewpoint article on CRISPR/Cas9-Based Functional Genomics in Human Induced Pluripotent Stem Cell–Derived Models
Identifier (DOI)
10.1002/mds.28998
Tags
  • Cas9
  • CRISPR
  • iPSCs (Induced pluripotent stem cells)
  • Review
Team
Team Vangheluwe
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

Related Research

CRISPR/Cas9 generation of knock-out iPSCs

This protocol describes RNP-based CRISPR/Cas9 gene-editing to generate knock-out iPSCs.

View Protocol

In vitro synthesis of PE2 (nCas9-MMLV RT fusion) polyA mRNA using T7 RNA polymerase

We describe the preparation of synthetic capped and polyadenylated messenger RNA (mRNA) encoding a Cas9 nickase-reverse transcriptase fusion protein (PE2) for use in genome editing with a method called prime editing.

View Protocol

Purification of the PE2 nCas9-RT protein

This protocol describes the process of expressing and purifying the nicking Cas9-MMLV RT fusion protein for prime editing.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.