CRISPR tagging of the TMEM192 gene with 3xHA in H9 ES AAVS-NGN2 cells for Lyso-IP

By Jiuchun Zhang, J. Wade Harper and Sharan Swarup, PhD
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Output Details

Description
This protocol describes a method for knockin of a 3X HA tag onto the C-terminus of the TMEM192 gene in human H9 ES AAVS1-NGN2 cells using CAS9 and a homologous recombination template
Identifier (DOI)
10.17504/protocols.io.e6nvwdjd7lmk/v1
Tags
  • CRISPR
  • Immunoprecipitation
  • Stem cells – embryonic
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

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