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Imaging of cholinergic interneurons in post-mortem rodent tissue to identify striatal satellite astrocytes

By Shinil Raina, MSc, Stephanie Cragg, PhD
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Output Details

Description
This protocol details confocal image acquisition steps and the analysis pipeline used to calculate inter-soma distance between cholinergic interneurons/pMSNs and their nearest astrocyte. The partner Protocol: Immunofluorescent Labelling of Post-Mortem Rodent Brain Tissue describes how to label cholinergic interneurons/pMSNs along with striatal astrocytes in PFA perfusion-fixed, 50-µm thick, post-mortem rodent brain tissue.
Identifier (DOI)
10.17504/protocols.io.kxygxypbzl8j/v1
Tags
  • Analysis
  • Astrocytes
  • Cell biology
  • Cholinergic neurons
  • Confocal microscopy
  • Microscopy - optical
  • Mouse
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

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