Immunocytochemistry of acute brain slices used in ex vivo voltammetry recordings

By Yanfeng Zhang, PhD and Stephanie Cragg, PhD
View Published Protocol

Output Details

Description
The steps detailed in this protocol are to label choline acetyltransferase (ChAT) or tyrosine hydroxylase (TH), which are known markers for cholinergic interneurons (Chl) and dopaminergic neurons and striatal axons (DA), respectively. We use an immunofluorescent approach in 300-µm thick slices of mouse brain tissue after performing ex vivo voltammetry recordings.
Identifier (DOI)
10.17504/protocols.io.n2bvj3jw5lk5/v1
Tags
  • Acetylcholine
  • Brain
  • Dopamine
  • Ex Vivo
  • Microscopy - optical
  • Mouse
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

Related Research

Custom Phyton scripts related to Bouabid, S. et al (2025) “Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations””

Phyton scripts written by Safa Bouabid and adapted from Han Lab for reading 2D treadmill ball velocity with Raspberry Pi.

View Code

Custom MATLAB scripts related to Bouabid, S. et al. (2025) “Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations”

MATLAB scripts written by Safa Bouabid to analyse ACh release dynamics, DA release, glutamate release onto cholinergic interneurons, and behavioural changes in mouse striatum during learning and extinction of Pavlovian associations.

View Code

Multi-fiber photometry in head-fixed mice performing a dual-cue delay Pavlovian association

Protocol describes dual-cue Pavlovian association & multi-fiber photometry in head-fixed mice. Mice trained to link cues with rewards & undergo extinction phase with reduced rewards.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.