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Measuring alpha-synuclein concentration in hiPSCs using ELISA

By Iona Thomas-Wright and Richard Wade-Martins, PhD
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Output Details

Description
This protocol, carried out according to manufacturer’s instructions uses a classical ELISA experimental design with sensitive electrochemical detection to provide measure of 𝜶-synuclein concentration in the conditioned media of cultured iPSC-derived human cells.
Identifier (DOI)
10.17504/protocols.io.rm7vzxb65gx1/v1
Tags
  • 2D cultures
  • Alpha-synuclein
  • Cell culture
  • ELISA
  • Expression
  • hiPSCs (Human induced pluripotent stem cells)
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

Related Research

Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”

The files found here are associated to Do, Q. et al (2023) study and contain: (a) all source and supplementary data, (b) Key Resources Table, and (c) list of primers.

View Dataset

Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation

The results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.

View Article

STC-1 cell culture

Cell culture of STC-1 mouse enteroendocrine cells.

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Aligning Science Across Parkinson's
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