Behavioral analyses in mice
By onThis collection of protocols describes different behavioural assays performed in mice including, grip strength test, pole test, rotarod, open field and nose poking learning task
ZSGreen x TH Colocalization Analysis
By onColocalization analysis of TH and ZSGreen using immunohistochemistry
Measuring lysosomal exocytosis by Flow cytometry
By onFlow cytometry can be used to measure lysosomal exocytosis by analyzing changes in fluorescence intensity of lysosomal markers in cells, providing a protocol for studying this cellular process.
Unbiased MD simulations of POPI entry into VPS34KD
By onThis protocol details molecular dynamics simulations of the kinase domain of VPS34 in a membrane containing 15% of POPI lipids and analysis of POPI entry into the catalytic site.
Image Processing and 3D Reconstruction
By onThis workflow was used to analyze a Krios dataset of the PI3KC3-C1/RAB1A Complex and generate a reconstruction of three distinct conformational states of the VPS34 lipid kinase domain.
Non-human Primate Arm-reaching Behavioral Tasks
By onProtocol for training and testing non-human primates in arm-reaching tasks to evaluate parkinsonian animals
Open Field & Cylinder test Behavior Assays
By onProtocols for mouse behavioral studies using the open field test and the cylinder test from Fushiki et al 2024.
Slow Release Pellet Implantation
By onThis protocol describes how to subcutaneously implant slow-release pellets into mice.
Western Blot with iBind System
By onProtocol used in the Gradinaru lab for western blot using the iBind Automated Western System
Immunostaining Mouse Tissue
By onProtocol used in the Gradinaru lab for immunohistochemistry to analyze protein expression in the mouse brain
Tissue Mito-IP: Immunopurification of Mitochondria from MitoTag Mice
By onA novel method called 'Mito-IP' enables rapid immunopurification of pure mitochondria in 10 minutes using a chimeric protein with HA tags, improving organelle isolation for various analyses.
Mito-IP: Immunopurification of Mitochondria from MitoTag Cultured Cells
By onA novel method called 'Mito-IP' enables rapid and pure mitochondrial isolation in 10 minutes, supporting various analyses. This technique is being extended to isolate other organelles like lysosomes, Golgi, and peroxisomes using epitope tags.
Analyses of mitochondrial function in SNpc with the Seahorse XFe96
By onProtocol for analyses of mitochondrial function in SNpc with the Seahorse XFe96
Western Blot – Ford Lab
By onThis protocol describes the solutions and steps for performing western blots.
Pipeline for Image Processing and Quantification of Striatal Dopamine Innervation using Fiji (IF/IHC)
By onThis protocol outlines the image preprocessing steps required prior to analysis with ImageJ tools as well as the ImageJ pipeline to quantify striatal innervation.
A CellProfiler computational pipeline to quantify localization of PPM1H on mitochondria
By onCellProfiler software pipeline quantifies PPM1H localization on fragmented mitochondria by hypotonic swelling, highlighting membrane contacts. Wild-type MEFs expressing PPM1H-mApple and GFP-Mito are imaged after treatment with oligomycin/antimycin.
Ex Vivo Electrophysiology V2 – Alternate version of Nelson lab protocol
By onThis protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.
3D spatial alignment of multiple similar objects extracted from FIB-SEM data using Fijiyama
By onProcedure for the use of Fijiyama ("Yet Another Matching and Alignment tool for Fiji") to spatially align a series of similar cellular structures detected from 3D FIB-SEM data.
Preparation of 3D distance probes for the quantification of variability in distance measurements between conditions
By onSimple procedure for preparing random 3D probe points for the analysis of image segmentation variability
