MSD V-PLEX Proinflammatory panel
By onThe V-PLEX panel offers analytically validated multiplex assay kits. Developed under rigorous design control, V-PLEX kits provide accurate and reproducible results with consistency from lot to lot.
Electrophysiology with iPSC-derived neurons
By onThis protocol describes the method to perform patch-clamp recordings of iPSC-derived neurons.
Protein structure prediction with AlphaFold-Multimer
By onThis protocol describes the protein structure prediction with AlphaFold-Multimer.
IHC and image analysis related to “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onIHC and image analysis related to "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Statistical analysis protocol for “”LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By onStatistical analysis protocol for ""LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration"
Model Building of PI3KC3-C1/RAB1A
By onDetails model building of the PI3KC3-C1/RAB!A complex using chimeraX1.5-1.6 and ISOLDE. Refinement was done using Phenix real space refinement.
Database-driven protein solubilization
By onThis is a protocol for using the extraction database found at www.polymerscreen.yale.edu for optimal extraction of membrane proteins into native nanodiscs.
Behavioral analyses in mice
By onThis collection of protocols describes different behavioural assays performed in mice including, grip strength test, pole test, rotarod, open field and nose poking learning task
ZSGreen x TH Colocalization Analysis
By onColocalization analysis of TH and ZSGreen using immunohistochemistry
Measuring lysosomal exocytosis by Flow cytometry
By onFlow cytometry can be used to measure lysosomal exocytosis by analyzing changes in fluorescence intensity of lysosomal markers in cells, providing a protocol for studying this cellular process.
Unbiased MD simulations of POPI entry into VPS34KD
By onThis protocol details molecular dynamics simulations of the kinase domain of VPS34 in a membrane containing 15% of POPI lipids and analysis of POPI entry into the catalytic site.
Image Processing and 3D Reconstruction
By onThis workflow was used to analyze a Krios dataset of the PI3KC3-C1/RAB1A Complex and generate a reconstruction of three distinct conformational states of the VPS34 lipid kinase domain.
Non-human Primate Arm-reaching Behavioral Tasks
By onProtocol for training and testing non-human primates in arm-reaching tasks to evaluate parkinsonian animals
Open Field & Cylinder test Behavior Assays
By onProtocols for mouse behavioral studies using the open field test and the cylinder test from Fushiki et al 2024.
Slow Release Pellet Implantation
By onThis protocol describes how to subcutaneously implant slow-release pellets into mice.
Western Blot with iBind System
By onProtocol used in the Gradinaru lab for western blot using the iBind Automated Western System
