QuBit HS RNA Quantification
By onQuBit HS RNA Quantification is a method used to accurately measure RNA concentration in samples with high sensitivity and precision.
Structural Analysis of 20S CPs and Assembly Intermediates by Electron Cryo-Microscopy
By onThis protocol details methods for structural determination by transmission electron cryo-microscopy of 20S CPs and assembly intermediates.
LC/MS analysis of plasma samples from PPMI
By onPlasma samples from PPMI were analyzed by liquid chromatography with mass spectrometry (LC/MS) for a variety of metabolites (including piperine) and lipids as interrelated markers of Parkinson's disease and its pathophysiology.
T cell differentiation from mice spleen tissue
By onThis protocol is for T cell differentiation from mice spleen tissue to investigate T cell function (including differentiation and proliferation) in vitro.
Single-Molecule Antibody Slides For Fluorescence Microscopy
By onThis protocol describes how to create single-molecule antibody slides for fluorescence microscopy.
Rating scale for parkinsonian motor signs in macaques and other non‐human primates
By onThis protocol details rating scale for Parkinsonian motor signs in macaques and other non‐human primates.
Sleep scoring using Neuroscore
By onStep by step instructions on how to analyze sleep data with Neuroscore v3.0.
Homology Modelling of Pacer RH domain in SWISS-MODEL
By onUsing the Rubicon RH - Rab7 crystal structure as a template (6wcw).
Behavior Hardware Setup Protocol
By onProtocol outlines steps for replicating conditioning/extinction reward learning assay.
In vivo Ephys: Analysis Protocol
By onProtocol outlines analysis steps for in vivo electrophysiology data after spike sorting.
In vivo Ephys: Spike Sorting Protocol
By onProtocol outlines spike sorting steps for electrode recording data.
Histology: Analysis Protocol
By onProtocol analyzes histology images using Matlab for defining ROIs and ilastik for pixel classification
Behavior: Analysis Protocol
By onThe protocol outlines how to visualize and analyze reward learning behavior data for research purposes.
Untargeted lipidomics of Tagless Lyso-IP
By onThe authors have developed the Tagless Lyso-IP method to rapidly prepare lysosome-enriched samples from human peripheral blood.
Untargeted metabolomics of Tagless Lyso-IP
By onLysosomal biology is increasingly implicated in neurodegenerative diseases and health. It has traditionally been difficult to profile the metabolomic homeostasis of the lysosome in disease states. To overcome this challenge, we have developed the Tagless Lyso-IP method to rapidly prepare lysosome enriched samples from human peripheral blood. This protocol details the processing and untargeted analysis of polar metabolites derived using the Tagless Lyso-IP method.
Fixation and Immunostaining protocol
By onFixation and immunostaining protocol for tissue and cell culture.
Light microscopy-based neuron tracing and reconstruction
By onProtocol outlines neuron reconstruction as delineating neuron components using light microscopy to create geometric models for quantitative morphology analysis.
