Quantifying the LAMP1 positive puncta
By onThis protocol describes quantifying the LAMP1 positive puncta.
Determination of NM Concentration
By onThis is the protocol for determining neuromelanin concentration and data.
Immunohistochemistry for Carbon Fiber Thread Electrodes
By onMethods for immunofluorescent staining of brain tissue with indwelling electrodes.
Immunofluorescent staining for neuronal marker MAP2
By onThis is the protocol for immunofluorescent staining for neuronal marker MAP2.
Proteomics workflow for APP/Aβ TOMAHAQ analysis in endosomal and lysosomal fractions
By onProtocol for analyzing Ab "half-tryptic" peptides from organelles using TOMAHAQ and Tomahto on a Thermo orbitrap instrument for detecting APP processing to Ab amyloid peptide.
LRRK2 Immunofluorescent staining
By onProtocol for immunofluorescent staining for LRRK2 in cultured cells using the MJFF2 (c41-2) antibody.
AT8 Tau Pathology Image Analysis
By onThis protocol describes how to analyze AT8 tau pathology in human brain tissue (FFPE sections with IHC).
Gene expression analysis by qPCR
By onProtocol for gene expression analysis by quantitative Real-Time PCR (qPCR).
Genotyping by next generation sequencing
By onThis collection describes a standard genotyping procedure using next generation sequencing (NGS) in the Hockemeyer lab.
CRISPResso analysis
By onThis protocol describes the process of using CRISPResso to analyze Next Generation Sequencing (NGS) data from CRISPR-targeted, genome-edited samples.
Automated Immunohistochemistry Staining
By onThe protocols describe the steps for automated immunohistochemistry staining using the Ventana Discovery® XT Immunostainer.
Acute striatal or midbrain fiber photometry in head-fixed mice
By onWhile this protocol focuses recordings from striatum with GCaMP, it can be easily modified to record from other brain regions and with other fluorescent reporters.
CD3 Cell Density in Substantia Nigra and Cerebral Peduncle Image Analysis
By onThis protocol describes how to measure CD3 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
In Silico analysis links the NSL complex to Parkinson’s disease and the mitochondria – Protein-protein interaction data to functional enrichment analysis
By onA bioinformatics approach taken to investigate the interactome of the NSL complex, to unpick its relevance to Parkinson’s disease progression.
Model building, validation, and visualization
By onProtocol describing Model building, validation, and visualization
