Protein interaction network analysis for Mendelian diseases
By onThis protocol describes the steps to use experimentally validated human data to create a protein-protein interaction network (PPIN) based on disease causative genes. Network analysis (combination of topological functional analyses) will lead to the identification of biological processes relevant for disease and disease endophenotypes.
Confocal imaging and digital image analysis
By onProtocol for confocal imaging on Nikon A1R microscope & digital image analysis is detailed.
Western blotting for LRRK2 signalling in macrophages
By onThis protocol describes the immunoblotting for components of the LRRK2 signalling pathway (LRRK2, LRRK2 pS935 and phospho-Rabs) using Invitrogen NuPage SDS-PAGE reagents and the BioRad Turbo Blot transfer system.
FACS-based enrichment of transfected hPSCs
By onThis protocol describes the procedure FACS-based enrichment of transfected human pluripotent stem cells (hPSCs).
Untargeted metabolomics analysis for Golgi immunopurification (Golgi-IP)
By onThis protocol provides details for analyzing GolgiIP metabolomics samples using liquid chromatography mass spectrometry (LC-MS) for polar metabolite profiling.
Golgi immunopurification (Golgi-IP) for subcellular metabolite profiling
By onThis protocol provides details for preparing Golgi-IP metabolomics samples.
Histology and Retention of Implanted Carbon Fiber Thread Electrodes in Fixed Tissue
By onMethods to cut and retain implanted carbon fiber electrodes in brain tissue.
Luminescent Conjugated Oligothiophenes (LCO) Staining
By onThe protocol describes Luminescent Conjugated Oligothiophenes staining on brain sections.
Create Mask for Axonal Quantification Analysis with FIJI
By onThis protocol describes how to create a quantified mask from an image of fluorescence-tagged axonal projections using FIJI/ImageJ software.
Untargeted lipidomics analysis for Golgi immunopurification (Golgi-IP)
By onThis protocol provides details for analyzing GolgiIP lipidomics samples using liquid chromatography mass spectrometry (LC-MS) for nonpolar lipid profiling.
Confirming circRNA expression by qPCR
By onThis protocol delineates a qPCR method to confirm the expression of circRNAs extracted from brain samples.
Protocol for Data Independent Acquisition – Mass spectrometry analysis – a DIA-based Organelle Proteomics
By onHere the authors provide a detailed protocol for the Data Independent Acquisition (DIA)-based mass spectrometry (MS) data acquisition method for proteomic profiling of the Golgi.
Catalepsy test (Bar test)
By onThe catalepsy test (bar test) was developed to test motor coordination and motor impairments.
Adhesive Removal Test to assess sensorimotor deficits in parkinsonian mice
By onThis behavior is used to assess fine motor movements in a mouse Parkinsonian model. It checks for correct paw and mouth sensitivity (time-to-contact) and correct dexterity (time-to-remove).
Ectopic receptor & endosome size and count image analysis and quantification
By onThis protocol is to help with the imaging analysis of ectopic receptor localization and endosome size.
Immunofluorescence Staining
By onThe protocol describes immunofluorescence staining on brain sections.
Immunohistochemistry Protocol – Chu lab (Wichman)
By onThis protocol describes immunohistochemistry.
Golgi immunopurification (Golgi-IP) for subcellular lipid profiling
By onThis protocol provides details for preparing Golgi-IP lipidomics samples.
