Quantification of Axonal Projections from Whole-Brain Slide Images
By onQuantification of Axonal Projections from Whole-Brain Slide Images provides a standardized method for processing and analyzing immunofluorescence images of mouse brain sections to quantify axonal projections.
A CellProfiler Pipeline for Quantification of p-SNCA in Mouse Striatal Cholinergic and Medium Spiny Neurons
By onBrain sections stained for pSNCA, DARPP-32, ChAT, and DAPI. Z-stacks processed into MIPs for analysis. Outputs include pSNCA intensity and neuronal mask area, normalised for cell type-specific burden using R script.
Whole-cell patch-clamp recordings
By onThis protocol describes the preparation of hippocampal slices from mice for whole-cell patch-clamp recordings and technique for whole-cell recordings from CA1 pyramidal neurons.
Measurement of Oxygen Consumption Rate (OCR) in iPSC-Derived Neurons and iPSCs Using Seahorse XFe96 Analyzer
By onProtocol measures OCR in iPSC-derived neurons & undifferentiated iPSCs using Seahorse XFe96 Analyzer.
Protein analyses and immunoblotting
By onProtocol for Western blotting includes cell lysis, protein quantification, SDS-PAGE, protein transfer, antibody incubation, and detection. Enables detection and quantification of target proteins.
Data Acquisition and Analysis of Derivatized Hexose/Glucose by LC-QQQ-MS
By onProtocol for analyzing derivatized hexose/glucose with LC-QQQ-MS using a C18 column and Ultivo mass spectrometer. Includes chromatographic conditions, MS settings, and data analysis for high-throughput quantification.
