Untargeted lipidomics analysis for Golgi immunopurification (Golgi-IP)
By onThis protocol provides details for analyzing GolgiIP lipidomics samples using liquid chromatography mass spectrometry (LC-MS) for nonpolar lipid profiling.
Confirming circRNA expression by qPCR
By onThis protocol delineates a qPCR method to confirm the expression of circRNAs extracted from brain samples.
Protocol for Data Independent Acquisition – Mass spectrometry analysis – a DIA-based Organelle Proteomics
By onHere the authors provide a detailed protocol for the Data Independent Acquisition (DIA)-based mass spectrometry (MS) data acquisition method for proteomic profiling of the Golgi.
Catalepsy test (Bar test)
By onThe catalepsy test (bar test) was developed to test motor coordination and motor impairments.
Adhesive Removal Test to assess sensorimotor deficits in parkinsonian mice
By onThis behavior is used to assess fine motor movements in a mouse Parkinsonian model. It checks for correct paw and mouth sensitivity (time-to-contact) and correct dexterity (time-to-remove).
Ectopic receptor & endosome size and count image analysis and quantification
By onThis protocol is to help with the imaging analysis of ectopic receptor localization and endosome size.
Immunofluorescence Staining
By onThe protocol describes immunofluorescence staining on brain sections.
Golgi immunopurification (Golgi-IP) for subcellular lipid profiling
By onThis protocol provides details for preparing Golgi-IP lipidomics samples.
Immunohistochemistry Protocol – Chu lab (Wichman)
By onThis protocol describes immunohistochemistry.
Quantifying the LAMP1 positive puncta
By onThis protocol describes quantifying the LAMP1 positive puncta.
Determination of NM Concentration
By onThis is the protocol for determining neuromelanin concentration and data.
Immunohistochemistry for Carbon Fiber Thread Electrodes
By onMethods for immunofluorescent staining of brain tissue with indwelling electrodes.
Immunofluorescent staining for neuronal marker MAP2
By onThis is the protocol for immunofluorescent staining for neuronal marker MAP2.
Proteomics workflow for APP/Aβ TOMAHAQ analysis in endosomal and lysosomal fractions
By onProtocol for analyzing Ab "half-tryptic" peptides from organelles using TOMAHAQ and Tomahto on a Thermo orbitrap instrument for detecting APP processing to Ab amyloid peptide.
LRRK2 Immunofluorescent staining
By onProtocol for immunofluorescent staining for LRRK2 in cultured cells using the MJFF2 (c41-2) antibody.
AT8 Tau Pathology Image Analysis
By onThis protocol describes how to analyze AT8 tau pathology in human brain tissue (FFPE sections with IHC).
Gene expression analysis by qPCR
By onProtocol for gene expression analysis by quantitative Real-Time PCR (qPCR).
