H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; PiggyBac-Keima-REEP5
By onES cells modified to produce iNeurons w/o ER-phagy receptor FAM134C/A/B, TEX264, and with Keima-REEP5 reporter. CRISPR/Cas9 used to introduce NEUROG2 construct in AAVS1 locus. Knockouts RETREG1/2/3, TEX264. Transfected with NEUROG2, REEP5, mKeima.
H9 ES AAVS1-NGN2 TEX264-/-
By onES cells modified using CRISPR/Cas9 lack ER-phagy receptor TEX264. They introduced a NEUROG2 construct in AAVS1 locus, derived from a female human embryonic stem cell at the blastocyst stage.
H9 ES AAVS1-NGN2 FAM134A-/-
By onSummary: H9 ES cells were modified using CRISPR/Cas9 to lack the ER-phagy receptor FAM134A. A NEUROG2 construct was introduced in the AAVS1 locus, creating iNeurons. The cells are derived from human embryonic stem cells at the blastocyst stage.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; CCPG1-/-
By onES cells were used to create iNeurons lacking ER-phagy receptors FAM134C, A, B, TEX264, and CCPG1. CRISPR/Cas9 was used to introduce a NEUROG2 construct in the AAVS1 locus. Knockouts included CCPG1, RETREG1, RETREG2, RETREG3, and TEX264.
H9 ES AAVS1-NGN2 FAM134B-/-; PiggyBac-Keima-REEP5
By onES cells modified to lack FAM134B and express Keima-REEP5 ER-phagy flux reporter were created using CRISPR/Cas9. They carry NEUROG2 construct in AAVS1 locus and are derived from human embryonic stem cells at blastocyst stage.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-
By onES cells were modified using CRISPR/Cas9 to lack ER-phagy receptor genes FAM134C, A, B, and TEX264. NEUROG2 construct was inserted into the AAVS1 locus. Cells were derived from human embryonic stem cells at the blastocyst stage.
A549 PPM1H-BromoTag (CVCL_C8YV)
By onCell Line: PPM1H-BromoTag CRISPR/CAS9 A549 knock-in cell line generated from parental A549 cells (ATCC A549 CCl-185) by introducing a BromoTag linked to the C-terminus of PPM1H using CRISPR/Cas9 gene-editing technology.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-; PiggyBac-Keima-REEP5
By onES cells were modified to create iNeurons lacking FAM134C, A, B receptors and expressing Keima-REEP5 ER-phagy flux reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct in AAVS1 locus and knockout RETREG1, 2, 3 genes.
H9 ES AAVS1-NGN2 FAM134C-/-; PiggyBac-Keima-RAMP4
By onES cells were modified using CRISPR/Cas9 to lack FAM134C and express Keima-RAMP4 ER-phagy flux reporter. NEUROG2 construct was introduced in AAVS1 safe harbor locus. Cells are embryonic stem cells derived from a female human at blastocyst stage.
HEK293 TMEM192-HA ::mNEON-YIPF4
By onHEK293 TMEM192-HA ::mNEON-YIPF4 HEK293::TMEM192-3xHA::mNEON-YIPF4 PubMed=37757899; Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Characteristics: Using CRISPR/Cas9 YIPF4 was N-terminally tagged on both alleles with mNeonGreen (PubMed=37757899). Transfected with: UniProtKB; A0A1S4NYF2; mNeonGreen (derivative of Branchiostoma lanceolatum blFP-Y3). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_C0I5 ! HEK293::TMEM192-3xHA Female Fetus Transformed cell line
HEK293T FBXO7-/-
By onHEK293T cells were genetically modified using CRISPR/Cas9 to delete the FBXO7/PARK15 gene.
H9 ES AAVS1-NGN2 CCPG1-/-
By onES cells modified using CRISPR/Cas9 lack the ER-phagy receptor CCPG1. NEUROG2 gene was introduced using a TRE3G promoter in AAVS1 locus. The cells are human embryonic stem cells derived from blastocysts.
H9 ES AAVS1-NGN2 FAM134C/A-/-; PiggyBac-Keima-REEP5
By onES cells were modified using CRISPR/Cas9 to express NEUROG2 and Keima-REEP5 for ER-phagy studies. Knockouts RETREG2 and RETREG3 were created, and cells were derived from a blastocyst stage female human embryonic stem cell line.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; CCPG1-/-; PiggyBac-Keima-RAMP4
By onES cells were used to create iNeurons lacking ER-phagy receptor genes FAM134C, A, B, TEX264, CCPG1. They expressed Keima-RAMP4 ER-phagy flux reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct in AAVS1 safe harbor locus.
H9 ES AAVS1-NGN2::TMEM192-3xHA YIPF4-/-
By onES cells modified to lack YIPF4 Golgi-phagy receptors were used to create iNeurons expressing NEUROG2. CRISPR/Cas9 was employed to introduce the construct in the AAVS1 locus of H9 ES cells.
H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; PiggyBac-Keima-RAMP4
By onES cells were modified to create iNeurons lacking ER-phagy receptors FAM134C, A, B, TEX264, and expressing Keima-RAMP4 ER-phagy flux reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct in AAVS1 locus and knockout RETREG and TEX264 genes.
H9 ES AAVS1-NGN2 CCPG1-/-; PiggyBac-Keima-REEP5
By onES cells were modified using CRISPR/Cas9 to lack CCPG1 and express Keima-REEP5 ER-phagy flux reporter. NEUROG2 construct was introduced in AAVS1 locus. Cells are embryonic stem cells derived from a human female blastocyst stage.
Tet-off TauRD (HEK293T)
By onAssociated with the following preprint: Saha et al., 2022 (published on biorxiv on Feb 19th, 2022) https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
iPS Cell line CRICKi011-A (iFCI016), c.G152A mutation in Exon 3 of SNCA
By onMutations in the SNCA gene cause rare autosomal dominant Parkinson’s disease. iPSC lines were created from individuals with SNCA G51D mutations, showing potential for disease modeling and therapy development for synucleinopathies.
