AAVS1-SA-neo-CAGGS-PE2-2A-GFP
By savannah onThis resource can be used to generate knock-in cell line faciliating prime editing.
pET30a(+)-PE2
By savannah onThis resource can be used to express and purify PE2 protein for RNP delivery.
pU6-pegRNA-HEK3-CTTins-px330-scaffold
By savannah onThis resource can be used to introduce HEK3 CTT insertion using prime editing. It serves as a good positive control for prime editing.
pU6-pegRNA-SNCA-A30P
By savannah onThis resource can be used to introduce SNCA-A30P mutation using prime editing.
pU6-pegRNA-SNCA-A53T
By savannah onThis resource can be used to introduce SNCA-A53T mutation using prime editing.
pCAG-MBP-Foldon-ATG9 (801-839)
By savannah onTo make ATG9 C-terminal tail trimer for expression in mammalian cells.
x4 GBA plasmids
By savannah onThe below plasmids are deposited and available via Addgene: https://www.addgene.org/Anthony_Schapira/. These have been used in publication: 10.1093/hmg/ddac233 188580 WT GBA pcDNA3.1 GBA (Homo sapiens) 188581 E326K GBA pcDNA3.1 GBA (Homo sapiens) 188582 L444P GBA pcDNA3.1 GBA (Homo sapiens) 188583 N370S GBA pcDNA3.1 GBA (Homo sapiens)
Tet-off TauRD (P301L/V337M)
By savannah onTet-off TauRD (P301L/V337M). Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
Tet-off TauRD (HEK293T)
By savannah onAssociated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
Tet-off Full Length Tau (0N4R, P301L/V337M)
By savannah onTet-off Full Length Tau (0N4R, P301L/V337M). Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
Tet-off FLTau (HEK293T)
By savannah onAssociated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
cDNA clone for bacterial expression of human PPM1H H153D
By quincy.tichenor onPlasmid for bacterial expression of human PPM1H (H153D); contains TEV protease site for removal of 6His tag.
cDNA clone for bacterial expression of human PPM1H D288A
By quincy.tichenor onPlasmid for bacterial expression of human PPM1H (D288A); contains TEV protease site for removal of 6His tag.
pBPK1520-HEK3-CTTins-ng
By quincy.tichenor onThis resource can be used to introduce HEK3 CTT insertion using prime editing, PE3 approach. It serves as a good positive control for prime editing.
pGST2-GST-TEV-NDP52
By quincy.tichenor onPlasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586
Px330-EGFP-LRRK2-CRISPR/Cas9
By quincy.tichenor onThis resource can be used to introduce LRRK2-G2019S mutation using CRISPR/Cas9 based genome editing.
pU6-pegRNA-LRRK2-G2019S-3a
By quincy.tichenor onThis resource can be used to introduce LRRK2-G2019S mutation using prime editing.
pU6-pegRNA-LRRK2-G2019S-3b
By quincy.tichenor onThis resource can be used to introduce LRRK2-G2019S mutation using prime editing.
pU6-pegRNA-LRRK2-G2019S-3c
By quincy.tichenor onThis resource can be used to introduce LRRK2-G2019S mutation using prime editing.