Lab Resource Archive

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  • pLKO.1 mTagBFP2 rat PGK1 shRNA

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    Expresses mTagBFP2 along with an shRNA against rat PGK1.

  • pLKO.1 mTagBFP2 rat DJ-1 shRNA

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    Expresses mTagBFP2 along with an shRNA against rat DJ-1.

  • KOLF2.1J DJ1 L166P 2

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    The iPSC lines used for the generation of the DJ1 L166P point mutation were from KOLF 2.1 J background (Pantazis et al., 2021).

  • KOLF2.1J DJ1 L166P 1

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    The iPSC lines used for the generation of the DJ1 L166P point mutation were from KOLF 2.1 J background (Pantazis et al., 2021).

  • pLenti HsATP13A2 WT (PAM) _ Hygro

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    Transfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT with hygromycin selection marker.

  • pLenti HsATP13A2 P652_E653 del

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    Transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del with puromycin selection marker.

  • ATG3 research outputs_discontinued

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    (https://docs.google.com/spreadsheets/d/1Wdx_852CaqRRkGTbrZTKnYch2eiRN74A/edit?usp=sharing&ouid=109625585762898855018&rtpof=true&sd=true) lists all ATG3 discontinued research outputs generated for https://www.biorxiv.org/content/10.1101/2023.07.17.549391v2 but not published in https://www.science.org/doi/10.1126/sciadv.adj8027 as they were deemed to be "not effective." These cell lines were replaced by another set published on the Hub.

  • pCAG-ATG13 (363-517)-MBP

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    Mammalian expression of ATG13 (363-517) with MBP tag.

  • pCAG-GST-ATG13 (363-460)

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    Mammalian expression of GST tagged ATG13 (363-460).

  • Calbindin KD mouse – Experimental cross

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    Transgenic mouse model with decreased calb1 expression in DAT-expressing cells.

  • Available ASAP-related hPSC collection from Team Studer

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    Collection of human pluripotent stem cell lines from Team Studer consisting of isogenic GBA, LRRK2, SNCA series across three genetic backgrounds, KI-reporter lines for TOMM20 for mitochondria, b-actin for cytoskeleton, LAMB1 for cell membrane and LAMP1 for lysosomes, a-synuclein overexpression lines for Lewy body-like aggregation models, and other hPSC resources.

  • HeLa S3 AZI2 KO clone 62

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    Cell Line from an African American population. Knockout cell: Method=CRISPR/Cas9; HGNC; 24002; AZI2.

  • HeLa S3 penta KO-ULK1/ULK2 DKO-AZI2/TBKBP1 DKO

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    Knockout cell: Method=CRISPR/Cas9; HGNC; 24002; AZI2. Knockout cell: Method=CRISPR/Cas9; HGNC; 29912; CALCOCO2. Knockout cell: Method=CRISPR/Cas9; HGNC; 6746; NBR1. Knockout cell: Method=TALEN; HGNC; 17142; OPTN. Knockout cell: Method=CRISPR/Cas9; HGNC; 11280; SQSTM1. Knockout cell: Method=CRISPR/Cas9; HGNC; 11575; TAX1BP1. Knockout cell: Method=CRISPR/Cas9; HGNC; 30140; TBKBP1. Knockout cell: Method=CRISPR/Cas9; HGNC; 12558; ULK1. Knockout cell: Method=CRISPR/Cas9; HGNC; 13480; ULK2.

  • HeLa S3 AZI2/TBKBP1 DKO clone 13

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    Cell Line from an African American population. Knockout cell: Method=CRISPR/Cas9; HGNC; 30140; TBKBP1.

  • HeLa S3 TBKBP1 KO clone 7

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    Cell Line from an African American population. Knockout cell: Method=CRISPR/Cas9; HGNC; 30140; TBKBP1.

  • HeLa S3 AZI2/TBKBP1 DKO clone 14

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    Cell line from an African American Population. Knockout cell: Method=CRISPR/Cas9; HGNC; 24002; AZI2. Knockout cell: Method=CRISPR/Cas9; HGNC; 30140; TBKBP1. Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.

  • HeLa S3 penta KO-AZI2/TBKBP1 DKO clone 20

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    Cell line from an African American Population. Knockout cell: Method=CRISPR/Cas9; HGNC; 29912; CALCOCO2. Knockout cell: Method=CRISPR/Cas9; HGNC; 24002; AZI2. Knockout cell: Method=CRISPR/Cas9; HGNC; 6746; NBR1. Knockout cell: Method=TALEN; HGNC; 17142; OPTN. Knockout cell: Method=CRISPR/Cas9; HGNC; 11280; SQSTM1. Knockout cell: Method=CRISPR/Cas9; HGNC; 11575; TAX1BP1. Knockout cell: Method=CRISPR/Cas9; HGNC; 30140; TBKBP1.

  • HeLa S3 penta KO-AZI2/TBKBP1 DKO clone 26

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    Cell line from an African American Population. Knockout cell: Method=CRISPR/Cas9; HGNC; 29912; CALCOCO2. Knockout cell: Method=CRISPR/Cas9; HGNC; 24002; AZI2. Knockout cell: Method=CRISPR/Cas9; HGNC; 6746; NBR1. Knockout cell: Method=TALEN; HGNC; 17142; OPTN. Knockout cell: Method=CRISPR/Cas9; HGNC; 11280; SQSTM1. Knockout cell: Method=CRISPR/Cas9; HGNC; 11575; TAX1BP1. Knockout cell: Method=CRISPR/Cas9; HGNC; 30140; TBKBP1.

  • pBMN-HA-NAP1

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    Plasmid: For mammalian expression of NAP1.

  • pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

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    Plasmid: For mammalian expression of NAP1 (delta-TBK1).

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