39S mitochondrial ribosome, cryo-ET STA from HeLa
By onSubtomogram average of the 39S mitochondrial ribosome, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
A 4-5X expansion microscopy protocol for high‑resolution imaging of olfactory sensory neuron cilia in mouse olfactory epithelium
By onProtocol for expanding mouse olfactory epithelium for improved imaging: OE dissected on a cold platform, embedded in hydrogel, and expanded 4-5 times with ExM. Enables detailed visualization of ciliary structures without super-resolution microscopy.
Microscopy-based bead assay
By onThis protocol describes the procedure to perform microscopy-based bead assay.
Microscopy-based pUb-coverage measurements of mitochondria in iNeurons
By onAssociated with preprint:
Live Imaging of Primary Mouse Neuron Cultures
By onThis protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging described involves labeling of dopamine neurons with a fluorescent DAT ligand and using virally encoded pHlourin sensors to measure vesicular release of neurotransmitter as the neurons are electrically stimulated. This technique was used in Jain et al., 2023 to compare vesicular release in neurons between various transgenic knockout mouse lines.
Evaluation of pUb kinetics using 3D-SIM
By onAssociated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1
Live imaging and analysis to investigate phase separation properties of NEMO during mitophagy
By onProtocol for live imaging and analysis to investigate phase separation properties of NEMO during mitophagy.
Immunocytochemistry of cultured human Medium Spiny Neurons (MSNs)
By onThis protocol describes the immunolabelling of one or several protein targets on PFA-fixed cell culture on glass coverslips.
Primary confocal microscopy data associated with the manuscript “Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B” (doi: 10.1101/2023.06.07.544051)
By onConfocal microscopy images, CellProfiler Excel files of Pearson's coefficients between TMEM55B/RILPL1 or pRab10/RILPL1 in R1441C MEF or VPS35 MEF cells, and Prism files of graphed Pearson's coefficients for each condition, as seen in doi: 10.1101/2023.06.07.544051.
Immunohistochemistry (IHC)
By onImmunohistochemistry for Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain.
Activation Induced Marker (AIM) Staining Protocol
By onThis protocol details about activation induced marker staining.
Damaged mitochondria recruit the effector NEMO to activate NF-κB signaling
By onDataset associated with manuscript "Damaged mitochondria recruit the effector NEMO to activate NF-κB signaling".
Fluorescence in situ hybridization (FISH)
By onFluorescence in situ hybridization (FISH) in Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain
Tomogram data of TauRD-Y forms amyloid-like aggregates in cells
By onTomograms: The tomograms shown in Fig. 1d and Fig. 3c are available in the EMDB https://www.ebi.ac.uk/emdb/ through the following information: EMD ID: EMD-13739 (Fig. 1d) and EMD-13740 (Fig. 3c)
Immunostaining Mouse Brain Tissue or Neuronal Cultures
By onThis protocol describes immunohistochemical and immunoctyochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for analysis.
Electron microscope sample preparation technique_V2
By onEM sample preparation protocol that is designed for processing cultured cell sample.
Cell culture, transfection, and imaging
By onThis protocol describes general procedures for culturing HeLa cells, transient transfection, and imaging using an Andor Dragonfly spinning disk confocal system.
Immunofluorescence imaging of cells expressing GolgiTAG (TMEM115-3HA) or HA-tagged non-Golgi annotated proteins
By onA method that can be used to verify the correct localisation of HA-tagged Golgi proteins by assessing their colocalization with known Golgi markers. olocalization with ACBD3.
