Protocols Suite — SH-SY5Y / iCell® Neurons / High-Content Imaging
By onMaintenance of SH-SY5Y cells with SNCA overexpression in DMEM/F-12, differentiation with RA/BDNF, and culture of iCell DopaNeurons with SNCA mutation. Treatment and immunostaining for downstream assays using specific protocols and analysis tools.
Microbiome signature of Parkinson disease in healthy and genetically at-risk individuals
By onParkinson's disease (PD) is disabling and costly. *GBA1* variants increase PD risk. Gut microbiome changes can predict PD progression and identify those at risk, suggesting potential for disease modification through nutrition.
Code R script related to doi.org/10.1038/s41531-025-01063-3
By onSevere GBA1 variants are linked to Parkinson's disease, impacting counseling and clinical trials. Access R script at doi.org/10.1038/s41531-025-01063-3 for more details.
Clinical data associated with doi.org/10.1038/s41531-025-01063-3
By onStudy shows severe *GBA1* variants contribute to Parkinson's Disease phenotype, impacting counseling and clinical trials. Findings available at doi.org/10.1038/s41531-025-01063-3.
Read Alignment table associated with doi.org/10.3389/fnins.2025.1546203
By onSummary: The publication explores the link between the *GBA1* host genotype and gut microbiome in the *GBA1*L444P/WT mouse model, shedding light on Parkinson's disease development.
MSP abundance table associated with doi.org/10.3389/fnins.2025.1546203
By onSummary: The table displays the abundance of microbial species in the gut of GBA1 L444P/WT mouse model, exploring the connection between GBA1 host genotype and gut microbiome in Parkinson's disease research.
Metagenomics sequencing data of publication doi.org/10.3389/fnins.2025.1546203
By onThe study explores the connection between *GBA1* gene variants and gut microbiome in a mouse model, shedding light on Parkinson's disease development.
ASAP CRN Cloud Release Notes – Version 4.0.0
By onASAP CRN Cloud released version 4.0.0 with expanded datasets including Human Postmortem-derived Brain Sequencing and Mouse datasets. New harmonized collections and individual datasets were added, enhancing research possibilities.
Myeloid PINK1 represses mtDNA release and immune signaling that impacts neuronal pathology in patient-derived idiopathic PD models
By onPINK1 represses mtDNA release and STING/NF-κB activation in peripheral macrophages of PD model laying the foundation for understanding PINK1-related peripheral mechanisms in idiopathic PD and providing targets for further therapy development
Protocols for “MAPL regulates gasdermin-mediated release of mtDNA from lysosomes to drive pyroptotic cell death”
By onThis work establishes a mitochondrial–lysosomal signaling axis in which MAPL-driven mtDNA export and gasdermin-mediated lysosomal permeabilization convert organelle stress into pyroptotic cell death.
Transcriptomics of primary bone marrow-derived macrophages from MAPL KO and control mice.
By onThis work establishes a mitochondrial–lysosomal signaling axis in which MAPL-driven mtDNA export and gasdermin-mediated lysosomal permeabilization convert organelle stress into pyroptotic cell death.
Protocols for “LRRK2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis.”
By onProtocols for "LRRK2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis."
Citrobacter rodentium Model of Enteropathogenic and Enterohemorrhagic (EPEC, EHEC) Escherichia coli Infection
By onPurpose: To outline preparation of inoculum and per os Citrobacter rodentium infection in mice.
