Plasma isolation from whole mouse
By onProtocol describing plasma isolation of adult mice. Used in Palumbos et al., 2025. Adapted from Goldsmith et al., 2022.
Immunocytochemistry of autophagosome-associated proteins
By onProtocol for immunocytochemistry used on primary cortical neurons in Palumbos et al., 2025. Protocol can be adapted to other cultured cell types and antibodies. Described protocol works for autophagosome associtated proteins
pCMV5D HA-PPM1H_M flap
By onCreating a hybrid protein by combining the HA-PPM1H protein with the flap domain of PPM1M.
Part2: PPM1M, a LRRK2-counteracting, phosphoRab12-preferring phosphatase with potential link to Parkinson’s disease
By onPublication seeks extra microscopy images for ChAT+ ciliation experiments, focusing on PPM1M, a phosphatase potentially linked to Parkinson's disease via LRRK2 and phosphoRab12.
Part1: PPM1M, a LRRK2-counteracting, phosphoRab12-preferring phosphatase with potential link to Parkinson’s disease
By onData and files for Western blot analysis and ciliation experiments on PPM1M, a potential Parkinson's disease-related phosphatase, are available for publication.
pFUW H2B-mCherry-P2A-PuroR
By onlentiviral expression construct: Expression and nuclear localization of mCherry
pFUW H2B-mEmerald-P2A-PuroR
By onlentiviral expression construct: Expression and nuclear localization of mEmerald
pFUW H2B-mTagBFP2-P2A-PuroR
By onlentiviral expression construct: Expression and nuclear localization of mTagBFP2
pFUW LAMP1Sig-mCherry
By onlentiviral expression construct: Expression and lysosomal localization of mCherry
pFUW LAMP1Sig-mEmerald
By onlentiviral expression construct: Expression and lysosomal localization of mEmerald
pFUW PuroR-P2A-COX8ASig-Timer
By onlentiviral expression construct: Expression and mitohcondrial localization of Timer
pFUW COX8ASig-mCherry
By onlentiviral expression construct: Expression and mitochondrial localization of mCherry
pFUW COX8ASig-mEmerald
By onlentiviral expression construct: Expression and mitochondrial localization of mEmerald
pFUW mito-GRX1-roGFP2
By onlentiviral expression construct: Expression and mitochondrial localization of Grx1-roGFP2
pFUW COX8ASig-Lemon
By onlentiviral expression construct: Expression and mitochondrial localization of Lemon, a pH biosensor
