Tomogram of aggregate in AgDD-sfGFP-expressing HEK293 cell 6 h post aggregation induction
By onAgDD protein aggregation in HEK293 cells can be temporally controlled for studying cellular effects. A cryo-electron tomogram shows AgDD aggregate formation 6h post-induction.
Cryo-EM map of in vitro grown G14R alpha-synuclein fibrils
By on3.0 A resolution cryo-EM map obtained from recombinant a-synuclein fibrils carrying the G14R mutation recently discovered in a patient of a complex neurological disease.
Cryo-EM map of in vitro grown WT alpha-synuclein fibrils
By onCryo-EM density map of recombinant WT a-synuclein fibrils at 2.7 A resolution.
Cryo-EM map of recombinant K58N alpha-synuclein fibrils
By onCryo-EM map obtained from recombinant a-synuclein fibrils carrying the K58N mutation recently discovered in a patient of Parkinson's disease.
Pathway and gates for ATG2A-mediated lipid transport in autophagy
By onATG2A ransfers lipids between donor and acceptor membranes leading to autophagosome formation. This study suggests that ATG2A is a lipid transporter gated at the N-terminus by blocking helices that, upon release, act as additional membrane tethers.
Reconstitution of multistep recruitment of ULK1 to membranes in autophagy
By onULK1C and PI3KC3-C1 work together to start autophagy. This work establishes a stepwise pathway for recruitment of the ULK1 KD to the vicinity of the membrane surface downstream of PI3KC3-C1 to initiate autophagy.
