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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Article
In situ structural analysis reveals membrane shape transitions during autophagosome formation
Preprint: A hallmark of PD is the failure of quality control mechanisms in the cell, such as autophagy. The authors combined cell biology with correlative cryo-electron tomography in yeast cells to show a high resolution stepwise structural progression of autophagosome biogenesis. Further, they revealed the organelle interactome for growing autophagosomes.
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Statistical analysis
This protocol describes the statistical analysis applied to the quantifications in Chang et al. 2021 SA paper.
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Article
Unconventional Initiation of PINK1/Parkin Mitophagy by Optineurin
Preprint: In this study, the authors untangle the mechanism behin OPTN in initating mitophagy, a process by which damaged mitochondria get degraded.
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GST Bead pulldown Assay
GST Pulldown Assay for recruitment of bait proteins to GST labeled prey proteins. Prey proteins can be purified or from lysate.
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Expression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs
Expression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs
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Expression and purification protocol of WIPI2d
This protocol details the expression and purification protocol of WIPI2d.
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Expression and purification protocol of GST-TAX1BP1
This protocol details the expression and purification protocol of GST-TAX1BP1.
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Expression and purification protocol of GST-mCh-FYVE
This protocol details the expression and purification of GST-mCh-FYVE.
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Expression and purification MBP-ATG9 Constructs
Expression and purification from HEK cells of ATG13, ATG101 and FOLDON-ATG9A proteins.
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Expression and purification GST-tagged ATG13:ATG101 constructs
Expression and purification of GST-tagged ATG13/ATG101 constructs.
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Electron microscope sample preparation technique_V2
There are eight main steps in preparing EM sample, including fixation, en bloc staining, agarose embedding, dehydration, infiltration, resin embedding, resin-embedded sample trimming and ultrathin sectioning. This EM sample preparation protocol is designed for processing cultured cell sample.
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