ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Preprint: A hallmark of PD is the failure of quality control mechanisms in the cell, such as autophagy. The authors combined cell biology with correlative cryo-electron tomography in yeast cells to show a high resolution stepwise structural progression of autophagosome biogenesis. Further, they revealed the organelle interactome for growing autophagosomes.
Preprint: In this study, the authors untangle the mechanism behin OPTN in initating mitophagy, a process by which damaged mitochondria get degraded.
Expression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs
This protocol details the expression and purification protocol of GST-TAX1BP1.
This protocol details the expression and purification of GST-mCh-FYVE.
Expression and purification of GST-tagged ATG13/ATG101 constructs.
There are eight main steps in preparing EM sample, including fixation, en bloc staining, agarose embedding, dehydration, infiltration, resin embedding, resin-embedded sample trimming and ultrathin sectioning. This EM sample preparation protocol is designed for processing cultured cell sample.