Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Article

In situ structural analysis reveals membrane shape transitions during autophagosome formation

Preprint: A hallmark of PD is the failure of quality control mechanisms in the cell, such as autophagy. The authors combined cell biology with correlative cryo-electron tomography in yeast cells to show a high resolution stepwise structural progression of autophagosome biogenesis. Further, they revealed the organelle interactome for growing autophagosomes.

Tool

pCAG-MBP-ATG9-P833A

Expresses human ATG9-P833A mutant in mammalian cells.

Protocol

Statistical analysis

This protocol describes the statistical analysis applied to the quantifications in Chang et al. 2021 SA paper.

Article

Unconventional Initiation of PINK1/Parkin Mitophagy by Optineurin

Preprint: In this study, the authors untangle the mechanism behin OPTN in initating mitophagy, a process by which damaged mitochondria get degraded.

Protocol

GST Bead pulldown Assay

GST Pulldown Assay for recruitment of bait proteins to GST labeled prey proteins. Prey proteins can be purified or from lysate.

Protocol

Expression and purification protocol of WIPI2d

This protocol details the expression and purification protocol of WIPI2d.

Protocol

Expression and purification protocol of GST-TAX1BP1

This protocol details the expression and purification protocol of GST-TAX1BP1.

Protocol

Expression and purification protocol of GST-mCh-FYVE

This protocol details the expression and purification of GST-mCh-FYVE.

Protocol

Expression and purification MBP-ATG9 Constructs

Expression and purification from HEK cells of ATG13, ATG101 and FOLDON-ATG9A proteins.

Protocol

Electron microscope sample preparation technique_V2

There are eight main steps in preparing EM sample, including fixation, en bloc staining, agarose embedding, dehydration, infiltration, resin embedding, resin-embedded sample trimming and ultrathin sectioning. This EM sample preparation protocol is designed for processing cultured cell sample.