pCDH1-CMV-FIP200-V44D/V314D-mCherry-Hygro
By onLentiviral expression vector encoding FIP200 mutant-mCherry
pCDH1-CMV-FIP200-V314D-mCherry-Hygro
By onLentiviral expression vector encoding FIP200 mutant-mCherry
pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro
By onLentiviral expression vector encoding FIP200 mutant-mCherry
pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro
By onLentiviral expression vector encoding FIP200 mutant-mCherry
His-GST-ATG13 (363-517)-ULK1 (836-1050)
By onBacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex
pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)-msfGFP
By onMammalian expression of TwinStrep-Flag -ATG13 C-terminal and ULK1 MIT domain fusion complex fused with GFP-10His
Mass photometry source data of Figure6
By onMass photometry source data of 'Fig. 6: Concentration-dependent stoichiometry shift of ULK1C' for DOI:2023.06.01.543278
Copy of MBP-ATG13 (363-517)-ULK1 (836-1050)-His
By onBacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His
MBP-ATG13 (363-517)-ULK1 (836-1050)-His
By onBacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His
Determining the effects of mecamylamine in the mouse striatum using a Conditioned Preference Place (CPP) paradigm
By onProtocol tests mecamylamine effects in mouse striatum using CPP paradigm to measure preference for drug-associated environment.
Intracranial injections of viral vectors in mouse midbrain and striatum
By onProtocol for injecting viral vectors in mouse midbrain and striatum is provided.
Immunocytochemistry of acute brain slices used in ex vivo voltammetry recordings
By onProtocol labels ChAT for cholinergic interneurons and TH for dopaminergic neurons in mouse brain slices. Immunofluorescence used post ex vivo voltammetry recordings on 300-µm thick slices.
In vivo voltammetry and fiber photometry in the mouse striatum
By onProtocol measures dopamine and calcium levels in striatal dopaminergic axons in vivo via voltammetry and fiber photometry in response to electrical stimulation.
Wide-field imaging of voltage sensors expressed in ex vivo mouse brain slices
By onThis protocol describes how to perform wide-field imaging of voltage sensors using high frame rates (660 Hz minimum every 2.5 minutes) in mouse midbrain using ex vivo brain slices.
Imaging axonal calcium dynamics in ex vivo mouse brain slices
By onProtocol for imaging calcium dynamics in striatal dopaminergic axons in ex vivo mouse brain slices using GCaMP6f in DAT-Cre:Ai95D mice. Calcium transients were observed in response to single and train electrical stimuli.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices following electrical or optogenetic stimulations
By onThis protocol is to assess changes in extracellular dopamine concentration following electrical or optogenetic stimulations in ex vivo mouse brain slices.
Datasets and Key Resources Table used in Zang, Y. et al. (2025) “An axonal brake on striatal dopamine output by cholinergic interneurons”
By onThis repository contains a 1) Key Resources Table with details on key resources and the persistent identifiers for protocols and code used and generated in this study; 2) Source Data Folder, and 3) Supplementary Data Folder.
Microinvasive Probes for Monitoring Electrical and Chemical Neural Activity in Nonhuman Primates
By onWe designed carbon fiber sensors that enable long-term simultaneous recording of both neurochemical and electrical neural activity in deep brain structures of nonhuman primates.