iNeuron differentiation from human iPSCs

By Dan Dou, Alexander Boecker, MD and Erika Holzbaur, PhD
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Output Details

Description
We adapted a previously-described method (Boecker et al., 2020, 2021; Fernandopulle et al., 2018) for differentiating iPSCs stably expressing mNGN2 at a safe-harbor locus into human excitatory glutamatergic neurons. Pre-i 3Neuron iPSCs (human iPSCs with an integrated doxycycline-inducible mNGN2 transgene in the AAVS1 safe-harbor locus) were a gift from M. Ward (National Institutes of Health, Maryland).
Identifier (DOI)
10.17504/protocols.io.261ge348yl47/v1
Tags
  • iPSCs (Induced pluripotent stem cells)
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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