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Preparing primary sandwich hippocampal neuron cultures for cryo-electron tomography

By Thanh Thao Do, Anna Siegert, MSc, Arsen Petrovic, PhD, Ruben Fernandez-Busnadiego, PhD, Florelle Domart and Thomas Dresbach
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Output Details

Description
Primary sandwich hippocampal neuron cultures facilitate targeting of neurons for cryo-electron tomography thanks to low cell density and almost no glial cells.
Identifier (DOI)
10.17504/protocols.io.6qpvr3y83vmk/v1
Tags
  • Cryo-ET
  • In Vitro
  • Microscopy - electron
  • Rat
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • User avatar fallback logo

    Thanh Thao Do

    External Collaborator

  • User avatar fallback logo

    Anna Siegert, MSc

    Key Personnel: Team Harper, 

    Universitätsmedizin Göttingen

  • User avatar fallback logo

    Arsen Petrovic, PhD

    Key Personnel: Team Harper, 

    Max Planck Institute of Biochemistry

  • Ruben Fernandez-Busnadiego, PhD

    Co-PI (Core Leadership): Team Harper, 

    University of Gottingen

  • User avatar fallback logo

    Florelle Domart

    External Collaborator

  • User avatar fallback logo

    Thomas Dresbach

    External Collaborator

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AgDD protein aggregation can be controlled for cellular studies. A cryo-electron tomogram shows an AgDD aggregate in a HEK293 cell 10 min post-aggregation induction.

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Tomogram of aggregate in AgDD-sfGFP-expressing HEK293 cell 6 h post aggregation induction

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Aligning Science Across Parkinson's
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