Staining of cells with GolgiTracker for Golgi flow cytometry analysis

By Enrico Bagnoli, PhD and Rotimi Fasimoye, PhD
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Output Details

Description

We describe a method that allows the staining of intact Golgi using GolgiTracker for subsequent flow cytometry analysis. The analysis can provide a quantitative indication of the level of enrichment of the organelle immunoprecipitation (% of GOLGI+ beads) and can be used as a quality control check for the purity of the immunoprecipitated Golgi.

Identifier (DOI)
10.17504/protocols.io.e6nvwk1d2vmk/v1
Tags
  • Flow cytometry
  • Golgi apparatus
  • Immunoprecipitation
Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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