CRISPR tagging of the TMEM192 gene with 3xHA in H9 ES AAVS-NGN2 cells for Lyso-IP
By onProtocol for adding a 3X HA tag to TMEM192 gene in human cells using CAS9 and recombination template is described for H9 ES AAVS1-NGN2 cells.
Expansion and maintenance of human induced pluripotent stem cells (iPSCs)
By onThis protocol describes the maintenance and expansion of iPSCs in the adherent culture via single-cell passaging.
Coating plates
By onThis protocol describes the process of coating plates using either VTN, Matrigel or Geltrex for use in culturing of feeder-free human pluripotent stem cells (hPSCs)
Feeder-free culturing of hPSCs
By onThis collection describes the standard procedure of feeder-free culturing of human pluripotent stem cells (hPSCs) using mTeSR-plus or StemFlex
Generation of ATG3 KO Hela cells stably expressing HaloTag-LC3B
By onThis protocol details generation of ATG3 KO HeLa cells stably expressing HaloTag-LC3B.
Thawing, Passaging and Freezing of hPSCs on MEFs
By onThis collection contains protocols which describe the standard procedure of culturing human pluripotent stem cells (hPSCs) on inactivated mouse embryonic fibroblasts (MEFs).
Subcloning of genotype-confirmed hPSCs clones
By onThis protocol describes a standard procedure for subcloning of genotype-confirmed human pluripotent stem cells (hPSCs).
Preparing mRNA for nucleofection of hPSCs
By onThis protocol describes the standard procedure for preparing mRNA to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.
CRISPR/Cas9 generation of knock-out iPSCs
By onThis protocol describes RNP-based CRISPR/Cas9 gene-editing to generate knock-out iPSCs.
iPSC differentiation into Macrophages
By onThis protocol describes iPSC differentiation into macrophages.
Culture and transfection of HEK293T cells
By onThis protocol describes a standard procedure culturing and transfecting HEK293T cells Protocol overview: A. Culturing HEK293T cells B. Transfection of HEK293T cells with Lipofectamine 2000
Midbrain organoid differentiation in spinner flasks
By onMidbrain differentiation protocol using spinner flasks
Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs
By onThis collection describes the standard procedure for the delivery of plasmids, mRNA or ribonucleoprotein (RNP) into human pluripotent stem cells (hPSCs) using nucleofection or electroporation.
Electroporation of hPSCs
By onThis protocol describes the standard procedure for the delivery of plasmids into human pluripotent stem cells (hPSCs) using electroporation.
Microglia differentiation from human pluripotent stem cells
By onMicroglia are crucial for brain development and maintaining homeostasis. They are involved in neurodegenerative diseases and can be generated from human stem cells using established protocols.
Generation of knock-in and knock-out CRISPR/Cas9 editing in mammalian cells
By onThis protocol is to help with generation of knock-in and knock-out CRISPR/Cas9 editing in mammalian cells
Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
By onThis protocol described the production of human cortical neurons from induced pluripotent stem cells in cocultured with commercially available rat astrocytes.
Generation of induced pluripotent stem cells and gene correction
By oniPSC generation and gene correction (CRISPR-CAS9) protocol.
Astrocyte isolation and culturing
By onThis protocol details steps for preparing an astrocyte monoculture from mouse pups. From dissection to plated samples ready to be transfected, treated, lysed, or imaged, the protocol takes 20-25 days.
