LRRK2 kinase inhibition restores haloperidol effects on rpS6 signaling in iSPNs
By onIF images in LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
LRRK2 disrupts the number and composition of active zone release sites in vulnerable dopamine axons
By onStriatal brain sections were stained with antibodies against bassoon to analyze the number and organization of active zone sites in Aldh1a1+ (EYFP+) or Aldh1a1- (mCherry+) axons from the same mouse
TH fibers and dopamine vesicle clustering alterations
By onTh fibers and dopamine vesicle clustering alterations in Lrrk2WT and Lrrk2G2019S mice
LRRK2 is expressed in dopamine neurons and is enriched in the vulnerable subpopulation
By onConfocal images from Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Striatal sections immunofluorescence and analysis
By onStriatal sections immunofluorescence and analysis related to LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
IBA1 Immunochemistry on human paraffin sections
By onIba1-specific Immunochemistry protocol on paraffin-embedded human brain sections
Olympus Slideview VS200″ slide scanning
By onBrief guide on the main steps to follow in order to scann a sample with the "Olympus Slideview VS200 slide scanner. Although examples for certain parameters are presented, each user should choose them based on the characteristics of their samples.
Datsets of Article Navigation Journal Article Longitudinal neuromelanin changes in prodromal and early Parkinson’s disease in humans and rat model
By onDatsets of Article Longitudinal neuromelanin changes in prodromal and early Parkinson’s disease in humans and rat model
MRI Datarat brain images used in “Longitudinal neuromelanin changes in prodromal and early Parkinson’s disease in humans and rat model”
By onThis Dataset contains rat brain images used in "Longitudinal neuromelanin changes in prodromal and early Parkinson’s disease in humans and rat ).
Standardised TruAI automated quantification of intracellular neuromelanin granules in human brain tissue sections
By onNeuromelanin (NM) granules were analysed in human post-mortem formalin-fixed paraffin embedded (FFPE) midbrain sections.
Flow cytometry data related to ‘The lipid flippase ATP10B enables cellular lipid uptake under stress conditions’
By onFlow cytometry data related to 'The lipid flippase ATP10B enables cellular lipid uptake under stress conditions'
Histology Albarran et al 2025
By onHistology protocol for Albarran et al 2025 that was adapted from Fushiki et al 2024 , to quantify Th+ cells.
Single particle analysis datasets of in vitro grown aSyn filaments
By onDataset includes cryo-EM micrographs of a-Synuclein fibrils with WT and mutant forms (G14R, K58N) found in patients.
Single particle analysis of α-Synuclein fibrils
By onAggregates of α-Synuclein are linked to Parkinson’s, multiple system atrophy, and Lewy body dementia. This protocol outlines near-atomic resolution structures of these amyloid fibrils using cryo-EM single particle analysis.
Preparing primary sandwich hippocampal neuron cultures for cryo-electron tomography
By onSandwich hippocampal neuron cultures have low cell density and few glial cells, making them ideal for targeting neurons for cryo-electron tomography.
Cryo-electron tomography of thinned synapses
By onProtocol for cryo-ET of thinned synapses in rat neuron cultures is detailed. Cryo-FIB milling creates thin samples for high-resolution imaging. Synapse targeting with and without cryo-FLM correlation is explained.
Albarran et al Histology dataset
By onDataset from a whole-brain imaging experiment showing recombinase expression in different brain regions using knock-in lines (D1-Cre, A2a-Cre, D1-FlpO, A2a-FlpO, DAT-FlpO) crossed with fluorescent reporter lines.
Protocols Suite — SH-SY5Y / iCell® Neurons / High-Content Imaging
By onMaintenance of SH-SY5Y cells with SNCA overexpression in DMEM/F-12, differentiation with RA/BDNF, and culture of iCell DopaNeurons with SNCA mutation. Treatment and immunostaining for downstream assays using specific protocols and analysis tools.
Sample Preparation Correlative XPCT – Histology
By onThis protocol describes the placement of samples for XPCT and preparation of correlative histological analysis after the measurement.
Replication Data for: 3D imaging of neuronal inclusions and protein aggregates in human neurodegeneration by multiscale X-ray phase-contrast tomography
By onDataset from DESY, GINX Beamline, Germany offers X-ray phase-contrast tomography volumes of human brain tissue for 3D analysis and reproducible imaging workflow correlation with histological sections.
