Primary Tabular Data for article “CHCHD2 mutant mice link mitochondrial deficits to PD pathophysiology” by Liao et al
By onMitochondrial dysfunction is linked to CHCHD2 mutation, causing altered protein interactions, glycolysis, and ROS, plus α-synuclein aggregation, and neurodegeneration. CHCHD2 accumulation leads to mitochondrial impairment and PD progression.
An ImageJ/FIJI Preprocessing Workflow for Multi-Series Confocal Microscopy Datasets Prior To CellProfiler Analysis
By onA workflow using ImageJ/FIJI aims to create three-slice MIPs from multi-series .czi files to capture weak signals. It involves extracting datasets, creating projections, and saving images for efficient analysis in CellProfiler.
Fluorescence recovery after photobleaching (FRAP) in neurons
By onThis protocol describes a fluorescence recovery after photobleaching (FRAP) assay used to measure the mobility of α-synuclein in synaptic boutons of cultured hippocampal neurons.
Immunofluorescence staining of cultured mouse hippocampal neurons
By onThis protocol describes the immunofluorescence staining of cultured neurons.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_ROS_Flow_SH-SY5Y
By onCellROX used to measure reactive oxygen species in SH-SY5Y wildtype, SLC45A1-KO, and rescued cells.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_LAMP1_Flow_SH-SY5Y
By onFlow cytometry was used to measure LAMP1 intensity in wildtype and SLC45A1-KO SH-SY5Y cells.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_TEM_Cortex
By onAnalysis of mouse cortex using transmission electron microscopy compared Slc45a1 wild-type and knockout samples. Data available on Zenodo.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_LAMP1_Primary neuron
By onStudy comparing LAMP1 signal in neurons from normal and Slc45a1 knockout mice using immunofluorescence analysis for quantification.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_TEM_SH-SY5
By onThe text mentions TEM SLC45A1 in SH-SY5Y cells, as shown in Figure 5B and S5Q.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Lamp1 staining_SH-SY5Y
By onFigure 5A shows Lamp1 staining in SLC45A1 knockout SH-SY5Y cells.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Characterization of GFP-SLC45A1 expression in U2OS.
By onSLC45A1 and Lamp1 co-localize in iNeurons at day 21 in vitro as shown in Figure 4E.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Colocalization of Flag-SLC45A1 with LAMP1_iNeuron D21
By onSLC45A1 and Lamp1 co-localize in iNeurons at day 21 in vitro as shown in Figure 4E.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Colocalization of Flag-GFP-SLC45A1 with LAMP1_SH-SY5Y
By onCo-localization of SLC45A1 and Lamp1 was observed in SH-SY5Y cells, as shown in Figure 4C.
ER mRFP-KDEL colocalization with GFP-tagged lysosomal candidates.
By onStudy examines colocalization of ER marker mRFP-KDEL with GFP-tagged lysosomal proteins to understand subcellular localization in cells.
Mitotracker colocalization with GFPtagged LysoIP enriched candidates.
By onMitotracker colocalizes with GFP-tagged LysoIP enriched candidates, indicating potential interaction between mitochondria and lysosomes.
Lysotracker colocalization with GFPtagged LysoIP enriched candidates.
By onLysotracker colocalization was observed with GFP-tagged LysoIP enriched candidates.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Image of Cre lines_Ghoochani A, Heiby J, et al,. 2025
By onImmunofluorescence staining of brain sections used to check colocalization of LysoTag with LAMP1, a marker for lysosomes.
Protocols for “MAPL regulates gasdermin-mediated release of mtDNA from lysosomes to drive pyroptotic cell death”
By onThis work establishes a mitochondrial–lysosomal signaling axis in which MAPL-driven mtDNA export and gasdermin-mediated lysosomal permeabilization convert organelle stress into pyroptotic cell death.
Protocol for Mouse Brain and SH-SY5Y Cell Sample Preparation for Electron Microscopy
By onProtocol for preparing mouse cortical tissue and SH-SY5Y cells for EM is outlined.
