Immunohistochemistry using paraffin embedded tissue
By onImmunohistochemistry using paraffin embedded tissue
Stereotaxic rat brain surgery for Substantia Nigra targeting
By onProtocol for stereotaxic rat brain surgery for Substantia Nigra targeting.
Endosome isolation
By onSubcellular fractionation to isolate early and late endosomes (EEs and LEs) by performing a series of centrifugation steps.
Nuclei Isolation and Sorting from Frozen Human Temporal Cortex V2
By onProtocol focuses on isolating and sorting nuclei from frozen human temporal cortex.
Perfusion-fixation procedure for adult rhesus monkeys
By onThe protocol details the perfusion-fixation procedure for rhesus monkeys
Standard Operating Procedure: Mouse Stereotaxic Intracranial Injection Surgery
By onSOP outlines procedures for mouse stereotaxic intracranial injection surgery.
Purification and Crystallization of ATG9 HDIR-ATG101:ATG13 complex
By onPurification and Crystallization of ATG9 HDIR (828-839) fused ATG101 (1-198):ATG13 (1-197).
Preparing of genomic DNA from in vitro cultured cells
By onThis protocol describes a standard procedure for preparing crude cell lysate which can be further analyzed by PCR.
AAV viral DNA and whole RNA recovery for AAV pool experiments in rhesus macaque
By onThis protocol outlines procedures to extract viral DNA and whole RNA from rhesus macaque tissue that had been treated with AAV in vivo
Post mortem human substantial nigra TH staining
By onThis protocol is standardized for postmortem (frozen) SN tissue from pathologically diagnosed PD patients and control individuals for Immunofluorescence staining.
Crystallization, data collection and structure solution of PxP-VAB
By onProtocol lists the steps to obtain PxP(Mcp1ct)-VABct crystals, X-ray diffraction data acquisition and obtaining the structure solution using molecular replacement with a model generated by AlphaFold2.
Preparing plasmids for nucleofection of hPSCs
By onThis protocol describes the standard procedure for the preparation of plasmids to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.
Cell lysis and immunoblotting for protein and phospho-protein quantification
By onThe authors describe the procedure by which human iPSC-derived neurons or mouse embryonic fibroblasts (MEFs) were lysed and probed for levels of proteins of interest using Western blot.
Tissue handling for mice, marmoset, and rhesus macaques injected with AAV
By onThis protocol outlines all procedures for handling of tissue of animal subjects treated with AAV.
Neural differentiation on EM grids – iNeurons sample preparation for cryo-ET and CLEM
By onProtocol for direct differentiation of AAVS1-TRE3G-NGN2 iPSCs and hESCs to iNeurons on EM grids: coat grids, seed iNeurons, transduce with fluorescent proteins, then plunge freeze for cryo-ET and cryo-CLEM.
Cryo-EM sample preparation
By onThis protocol details how to prepare cryo-EM samples of 3xFLAG-SHIP164Δ901-1099
Striatal dopamine measurement through HPLC
By onProtocol for striatal dopamine measurement in mouse brain
