An ImageJ/FIJI Preprocessing Workflow for Multi-Series Confocal Microscopy Datasets Prior To CellProfiler Analysis
By onA workflow using ImageJ/FIJI aims to create three-slice MIPs from multi-series .czi files to capture weak signals. It involves extracting datasets, creating projections, and saving images for efficient analysis in CellProfiler.
Endogenous N-terminal 3x FLAG Tagging of SLC45A1 in iPSCs
By onProtocol outlines tagging SLC45A1 gene N-terminal with 3X FLAG tag in WTC11-Ngn2 iPSCs using Cas9-sgRNA RNP complex & ssODN template. Allows point mutations or inserting sequences up to 130 bp, inserting 66 bp 3X FLAG tag after start codon.
Adherent mammalian Cell Culture maintenance
By onSubculturing involves transferring cells into fresh growth medium to maintain healthy cell cultures. It is a crucial step in the propagation of HEK293T, SH-SY5Y, and U2OS cell lines.
BrainLysoAtlas
By onSummary: Research by Ghoochani et al. presents a code repository linked to their study on brain lysosomes, identifying SLC45A1-related disease as a lysosomal disorder.
MS LysoIP SH-SY5Y
By onNovel lysosomal proteins in brain cells linked to disease. Proteomic changes in lysosome upon SLC45A1 KO in fed/starved conditions and whole cell proteomic changes are studied. Note: Raw file names may not match actual conditions.
MS BioID
By onStudy discovers new lysosomal proteins in brain cells, some associated with diseases. Neuron-specific protein SLC45A1 linked to neurological disease. Interaction partners of SLC45A1 and TMEM192 identified.
MS LysoIP mouse
By onMass spectrometry analysis of LysoIP mouse tissue identified proteins involved in lysosomal functions, providing insights into cellular processes and potential therapeutic targets.
Lysosomal Brain Protein Atlas Interactive Browser
By onA web tool hosts a proteomic atlas for cell-type-specific queries on LysoIP protein abundance, comparisons, and annotations. Accessible at without login.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Seahorse data
By onMitochondrial oxygen consumption rate (OCR) test.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_ROS_Flow_SH-SY5Y
By onCellROX used to measure reactive oxygen species in SH-SY5Y wildtype, SLC45A1-KO, and rescued cells.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_pH measurement_LysoSensor Yellow/Blue_SH-SY5Y
By onThe study investigates lysosomal pH levels in wildtype and SLC45A1-KO SH-SY5Y cells. The data is available on Zenodo for further analysis.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_FIRE-pHLy_mFTP1/mCherry_SH-SY5Y
By onFIRE-pHLy reporter used to assess lysosomal acidity in SH-SY5Y cells.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Cathepsin B activity Assay
By onStudy uses live-cell imaging to measure Cathepsin B activity in wildtype, SLC45A1-KO, and rescued cells during serum deprivation.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_LAMP1_Flow_SH-SY5Y
By onFlow cytometry was used to measure LAMP1 intensity in wildtype and SLC45A1-KO SH-SY5Y cells.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_TEM_Cortex
By onAnalysis of mouse cortex using transmission electron microscopy compared Slc45a1 wild-type and knockout samples. Data available on Zenodo.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_LAMP1_Primary neuron
By onStudy comparing LAMP1 signal in neurons from normal and Slc45a1 knockout mice using immunofluorescence analysis for quantification.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_TEM_SH-SY5
By onThe text mentions TEM SLC45A1 in SH-SY5Y cells, as shown in Figure 5B and S5Q.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Lamp1 staining_SH-SY5Y
By onFigure 5A shows Lamp1 staining in SLC45A1 knockout SH-SY5Y cells.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Characterization of GFP-SLC45A1 expression in U2OS.
By onSLC45A1 and Lamp1 co-localize in iNeurons at day 21 in vitro as shown in Figure 4E.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Colocalization of Flag-SLC45A1 with LAMP1_iNeuron D21
By onSLC45A1 and Lamp1 co-localize in iNeurons at day 21 in vitro as shown in Figure 4E.
