Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Extracellular space diffusion modelling identifies distinct functional advantages of archetypical glutamatergic and GABAergic synapse geometries
We modelled extracellular diffusion in super-resolved images of live brain tissue neuropil. We found that extracellular space geometry shapes local diffusion and this has functional implications for signaling arising from synaptic spill-over.
ASAP CRN Cloud Release Notes – Version 2.0.0
Version: 2.0.0 Release Date: December 11, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 2.0.0 of the CRN Cloud platform.
ASAP CRN Cloud Release Notes – Version 1.0.0
Version: 1.0.0 Release Date: June 25, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 1.0.0 of the CRN Cloud platform.
ASAP CRN Cloud Release Notes – Version 1.0.0-beta
Version: 1.0.0-beta Release Date: March 6, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 1.0.0-beta of the CRN Cloud platform.
ASAP CRN Cloud Release Notes – Version 0.0.1
Version: 0.0.1 Release Date: November 10, 2023 This release notes document provides a concise overview of Version 0.0.1 of the CRN Cloud platform.
Dephosphorylation of phosphorylated Rab GTPases by PPM phosphatases
Protocol measures phosphoRab GTPase dephosphorylation with PPM phosphatases using purified phosphoRab GTPases. Substrates generated by phosphorylating Rab GTPases with MST3 kinase are included.
Flowcytometry analysis of lysosomal pulldown with anti-TMEM192 magnetic beads from PBMCs
Method described for staining intact lysosomes with lysotracker for flow cytometry. Analysis provides quantitative indication of organelle enrichment in immunoprecipitation, serving as quality control.
Sample Preparation for Proteomic Analysis of Isolated Mitochondria and Whole-Cell Extracts
Protocol outlines precise sample prep for mass spectrometry-based proteomics, emphasizing robust lysis, S-trap column protein capture, and optimized digestion for high-quality data on proteomic changes and post-translational modifications.
Mito-IP: Immunopurification of Mitochondria from MitoTag Cultured Cells
A novel method called 'Mito-IP' enables rapid and pure mitochondrial isolation in 10 minutes, supporting various analyses. This technique is being extended to isolate other organelles like lysosomes, Golgi, and peroxisomes using epitope tags.
GCase assay – Resorufin β-D-glucopyranoside
Method presented for assessing lysosomal enzyme GBA1 activity using Resorufin substrate in low pH conditions to minimize non-lysosomal activity. Validated in GBA1 KO cells, compatible with various lysates and adjustable for different GBA1 levels.
A CellProfiler computational pipeline to quantify localization of PPM1H on mitochondria
CellProfiler software pipeline quantifies PPM1H localization on fragmented mitochondria by hypotonic swelling, highlighting membrane contacts. Wild-type MEFs expressing PPM1H-mApple and GFP-Mito are imaged after treatment with oligomycin/antimycin.
Mouse brain immunohistochemistry – colorimetric analysis
Protocol for colorimetric analysis of mouse brain immunohistochemistry is described.
p-S65-Ub sandwich ELISA
Assay measures Ubiquitin phosphorylation at Serine-65 in vivo using ELISA. Method previously detailed by Watzlawik et al. in their publication.
PINK1 WT/LRRK2 WT, PINK1 WT/LRRK2 RC, PINK1 KO/LRRK2 WT, PINK1 KO/LRRK2 RC
Mice with LRRK2 R1441C and PINK1 KO mutations were crossed to create double mutant lines: LRRK2 WT/PINK1 WT, LRRK2 RC/PINK1 WT, LRRK2 WT/PINK1 KO, and LRRK2 RC/PINK1 KO.
LRRK2 R1441C/PINK1 Double mutant mouse embryonic fibroblasts
LRRK2 R1441C/PINK1 double mutant mouse embryonic fibroblasts were studied alongside homozygous mutant and littermatched wild-type cells.
