Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
L1-silenced iPSC line (HS1_L1HS-CRISPRi_g1)
Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS1_L1HS-CRISPRi_g2 )
Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line – Control (HS1_L1HS-CRISPRi_LacZ )
Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS2_L1HS-CRISPRi_g1)
Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS2_L1HS-CRISPRi_LacZ)
Human iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
α-Synuclein Promotes Neuronal Dysfunction and Death by Disrupting the Binding of Ankyrin to β-Spectrin
α-Synuclein plays a key role in the pathogenesis of Parkinson’s disease and related disorders, but critical interacting partners and molecular mechanisms mediating neurotoxicity are incompletely understood. We show that α-synuclein binds…
Neuromodulator control of energy reserves in dopaminergic neurons
Neurons, are vulnerable to metabolic changes. Dopamine helps regulate glycogen storage providing resilience to stress. Loss of this regulation can make dopamine neurons hypersensitive to fuel deprivation, potentially leading to neurodegeneration.
Structural basis for binding of RILPL1 to TMEM55B reveals a lysosomal platform for adaptor assembly through a conserved TBM motif
Inherited mutations in VPS35 and LRRK2 cause Rab GTPase hyperphosphorylation. TMEM55B on lysosomes interacts with RILPL1 through a specific motif, forming complexes with various proteins, revealing new insights into lysosomal function.
RNA scope for ATP10b
Protocol for RNAscope® in situ hybridization on fixed-frozen mouse brain tissue using the RNAscope® Multiplex Fluorescent v2 kit. Includes staining steps for dorsal striatum, sample preparation, and cryosectioning.
Integrated Representations of Threat and Controllability in the Lateral Frontal Pole
Emotions can strongly influence behavior, as seen in PD paradoxical kinesia. Here, we demonstrate that anticipating threats enhances motor accuracy through interactions between the amygdala and the anterior pole of the lateral prefrontal cortex.
Zhai et al. 2025 Raw data
Data includes immunohistochemistry images, two-photon images of patched neurons, whole-cell recordings for somatic excitability and Sr2+-oEPSC, spine density imaging, ACh sensor imaging, and dendritic excitability linescan data.
Autophagy detection assay
Autophagosome detection using Dojindo DAPRed dye and Lysotracker Green in neurons
Ubiquitin enrichment assay
Ubiquitin enrichment assay from neuronal lysates
Immunoprecipitation from primary neurons
Protocol for immunoprecipitating proteins from neuronal lysates
Assay for lysosomal activity using Magic Red
Assay for lysosomal Cathepsin B activity in neurons using Magic Red
