Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Synaptic location is a determinant of the detrimental effects of α-synuclein pathology to glutamatergic transmission in the basolateral amygdala
αSyn expression is restricted in a subset of glutamatergic synapses in BLA and its aggregation decreases cortico-BLA transmission through both gained toxicity and loss of normal function. These results might be relevant to the reduced cortical control of amygdala function that has been associated with psychiatric deficits in PD.
A topographical atlas of αSyn dosage and cell-type expression in the mouse brain and periphery
Published: Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide and presents pathologically with Lewy pathology and dopaminergic neuron loss. This atlas provides much-needed insight into the cellular topography of αSyn, and provides a quantitative map to test assumptions about the role of αSyn in network vulnerability in PD and other αSynucleinopathies. View original preprint.
Regional mouse brain analysis (Modified QUINT)
This series of protocols has been adapted from published and unpublished protocols broadly referred to as the QUINT workflow:
Note that the original QUINT workflow was generated by Yates and colleagues, and all credit for the development of these programs goes to that team.
Teams
Spatial transcriptomics reveals molecular dysfunction associated with Lewy pathology
Published: The results identify neurons vulnerable to Lewy pathology in the PD cortex and identify a conserved signature of molecular dysfunction in both mice and humans. View original preprint.
Teams
Vibrational stabilization of cluster synchronization in oscillator networks
Preprint: Deviations from normal cluster synchronization patterns are closely associated with various malfunctions, such as neurological disorders in the brain. Here, the authors employ an open-loop control strategy, vibrational control, which does not require any state measurements. Additionally, they conduct numerical experiments to demonstrate their theoretical findings.
Teams
Vibrational stabilization of complex network systems
Published: Many natural and man-made network systems need to maintain certain patterns, such as working at equilibria or limit cycles, to function properly. The authors provide some numerical results that demonstrate the validity of our theoretical findings.
Teams
LRRK2 kinase inhibition reverses G2019S mutation-dependent effects on tau pathology spread
Published: Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of familial Parkinson’s disease (PD). This work supports a protective role of LRRK2 kinase inhibition in G2019S carriers and provides a rational workflow for systematic evaluation of brain-wide phenotypes in therapeutic development. View original preprint.
Teams
Whole mount dissection and staining of enteric nervous system
This protocol details whole mount dissection and staining of the enteric nervous system.
Teams
Linear diffusion modeling of tau pathology spread
Code to reproduce all network analyses in Lubben et al.: “LRRK2 kinase inhibition reverses G2019S mutation-dependent effects on tau pathology spread.”
Teams
Embryonic/Postnatal mouse neuron culture protocol
This protocol details the primary neuron culture procedures from embryonic and postnatal mice.
Teams
α-Synuclein protein preparation (Large scale)
Protocol for large-scale preparation of recombinant α-synuclein protein.
Teams
Cortico-amygdala synaptic structural abnormalities produced by templated aggregation of α-synuclein
PD and DLB involve α-syn inclusions in the amygdala affecting cognition and emotions. Cortico-amygdala synapses with α-syn aggregates show increased volume of synapses, potentially contributing to behavioral impairments.
Teams
Direct ELISA
This protocol details how to set up and run a direct ELISA on your protein of interest.
Teams
Human brain sequential extraction (Tau)
This protocol details human brain sequential extraction for pathological tau.
This protocol is an adaptation of the work of several labs.
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