ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
RNA and protein extraction from bulk dissections
Protocol for RNA and protein extraction from bulk dissections.
Immunochemistry on paraffin sections
Protocol for immunochemistry on paraffin sections.
Gene expression analysis by qPCR
Protocol for gene expression analysis by quantitative Real-Time PCR (qPCR).
In vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease
Preprint: Using a newly developed rodent model, the authors assessed whether the intracellular buildup of neuromelanin that occurs with age can be slowed down in vivo to prevent or attenuate Parkinson’s disease. when neuromelanin reaches a specific threshold, it can trigger PD pathology in this animal model.
Cylinder behavioral test
Protocol for cylinder behavioral test for rats.
Stereotaxic rat brain surgery for Substantia Nigra targeting
Protocol for stereotaxic rat brain surgery for Substantia Nigra targeting.
Intracellular neuromelanin quantification
Protocol for intracellular neuromelanin quantification.
Rat brain processing for histological analyses
Protocol for rat brain processing for histological analyses.
Immunofluorescence on paraffin sections
Protocol for immunofluorescence on paraffin sections.
Determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method
Protocol for the determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method.
Protocol for cDNA synthesis.
Stereology-mediated cell counting using StereoInvestigator
Protocol for stereologic count with StereoInvestigator.