Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Protocol

Protocols for “Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice”

This is a collection of protocols associated to the manuscript “Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice.”

Article

The calcium sensor synaptotagmin-1 is critical for phasic axonal dopamine release in the striatum and mesencephalon, but is dispensable for basic motor behaviors in mice

The authors’ findings reveal the striking resilience of DA-dependent motor functions in the context of a near-abolition of phasic DA release, shedding new light on why extensive loss of DA innervation is required to reveal motor dysfunctions in PD.

Article

Parkinson’s genes orchestrate pyroptosis through selective trafficking of mtDNA to leaky lysosomes

Preprint: Breach of lysosomes allows mtDNA to access cytosol, requiring multiple Parkinson’s disease-related proteins and Gasdermin pores, identified in the screen. These data place mitochondria-to-lysosome transport as a driver of pyroptosis and link multiple PD proteins along a common pathway.

Dataset

Single cell transcriptomics from DAT-Cre mouse gut tissue

Colonic myenteric cells were dissociated, and live tdTomato-positive cells were FACS-collected. Colons were isolated from 2 male and female wild type adult DAT-Cre mice. Wild type C57Bl6/J was used as control for gating. Cells were processed according to 10X Genomics Chromium single cell 3′ Reagent guidelines.

Article

Microbes and Parkinson’s Disease: from associations to mechanisms.

Review: This review dissects the microbial contributions to PD with a particular focus on recent advances and rodent models that can be used for microorganism hypothesis testing.

Article

LRRK2 regulates the activation of the unfolded protein response and antigen presentation in macrophages during inflammation

Remarkably, the PD-related protein LRRK2 acted with STING upstream of the UPR to regulate the transition from innate to adaptive immunity, thereby identifying this PD-related protein as a key player in the immune response during inflammation.

Article

Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice

Given the clear heterogeneity of DA gut neurons, further investigation is warranted to define their functional signatures and discover their inherent biological differences that put these cells at risk for neurodegeneration.

Article

Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice

Taken together, the authors’ findings reveal the striking resilience of DA-dependent motor functions in the context of a near-abolition of phasic DA release, shedding new light on why extensive loss of DA innervation is required to reveal motor dysfunctions in PD.

Protocol

Protocols for “Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice”

This is a collection of protocols for the manuscript “Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice.”