Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
High-yield monocyte/macrophage differentiation from hiPSC
Here, the protocol describes a highly efficient method for differentiating monocytes/macrophages from hiPSC.
Teams
Adoptive transfer of mitochondrial antigen-specific CD8+ T-cells in mice causes parkinsonism and compromises the dopamine system
The degeneration of dopamine neurons in the ventral midbrain is linked to the development of motor symptoms in Parkinson’s disease (PD). Evidence suggests that neuroinflammation and mitochondrial dysfunction drive neurodegenerative mechanisms in PD. Results provide evidence for mitochondrial-specific CD8+ T cell infiltration in the brain in driving PD-like pathology.
Teams
The calcium sensor synaptotagmin-1 is critical for phasic axonal dopamine release in the striatum and mesencephalon, but is dispensable for basic motor behaviors in mice
The authors’ findings reveal the striking resilience of DA-dependent motor functions in the context of a near-abolition of phasic DA release, shedding new light on why extensive loss of DA innervation is required to reveal motor dysfunctions in PD.
Teams
Protocols for “Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice”
This is a collection of protocols for the manuscript “Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice.”
Teams
Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice
Taken together, the authors’ findings reveal the striking resilience of DA-dependent motor functions in the context of a near-abolition of phasic DA release, shedding new light on why extensive loss of DA innervation is required to reveal motor dysfunctions in PD.
Teams
Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice
Given the clear heterogeneity of DA gut neurons, further investigation is warranted to define their functional signatures and discover their inherent biological differences that put these cells at risk for neurodegeneration.
Teams
LRRK2 regulates the activation of the unfolded protein response and antigen presentation in macrophages during inflammation
Remarkably, the PD-related protein LRRK2 acted with STING upstream of the UPR to regulate the transition from innate to adaptive immunity, thereby identifying this PD-related protein as a key player in the immune response during inflammation.
Teams
Protocols for “Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice”
This is a collection of protocols associated to the manuscript “Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice.”
Teams
Single cell transcriptomics from DAT-Cre mouse gut tissue
Colonic myenteric cells were dissociated, and live tdTomato-positive cells were FACS-collected. Colons were isolated from 2 male and female wild type adult DAT-Cre mice. Wild type C57Bl6/J was used as control for gating. Cells were processed according to 10X Genomics Chromium single cell 3′ Reagent guidelines.
Teams
Parkinson’s genes orchestrate pyroptosis through selective trafficking of mtDNA to leaky lysosomes
Preprint: Breach of lysosomes allows mtDNA to access cytosol, requiring multiple Parkinson’s disease-related proteins and Gasdermin pores, identified in the screen. These data place mitochondria-to-lysosome transport as a driver of pyroptosis and link multiple PD proteins along a common pathway.
Teams
Comparative analysis of methods to reduce activation signature gene expression in PBMCs
Preprint: These findings highlight the importance of considering the advantages and drawbacks of different isolation methods to ensure accurate interpretation of PBMC transcriptomic profiles.
Teams
Mitochondrial antigen presentation in RAW macrophages
This protocol details methods for 3-day Mitochondrial Antigen Presentation Assay in a murine macrophage cell line (RAW) that expresses a glycoprotein B (gB) of herpes simplex virus 1 (HSV1) targeted to the mitochondrial matrix (mito-gB). Watch an interview about this protocol with Ahmed Fahmy, PhD.
Teams
Modeling gene-environment interactions in Parkinson’s disease: Helicobacter pylori infection of Pink1-/- mice induces CD8 T cell-dependent motor and cognitive dysfunction
Parkinson’s disease (PD) is a neurodegenerative disorder characterized by loss of motor function. Using a mouse model, the authors demonstrate that infection with Helicobacter pylori leads to the development of motor and cognitive signs.
Teams
Microbes and Parkinson’s Disease: from associations to mechanisms.
Review: This review dissects the microbial contributions to PD with a particular focus on recent advances and rodent models that can be used for microorganism hypothesis testing.
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