ER mRFP-KDEL colocalization with GFP-tagged lysosomal candidates.
By onStudy examines colocalization of ER marker mRFP-KDEL with GFP-tagged lysosomal proteins to understand subcellular localization in cells.
Mitotracker colocalization with GFPtagged LysoIP enriched candidates.
By onMitotracker colocalizes with GFP-tagged LysoIP enriched candidates, indicating potential interaction between mitochondria and lysosomes.
Lysotracker colocalization with GFPtagged LysoIP enriched candidates.
By onLysotracker colocalization was observed with GFP-tagged LysoIP enriched candidates.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder_Image of Cre lines_Ghoochani A, Heiby J, et al,. 2025
By onImmunofluorescence staining of brain sections used to check colocalization of LysoTag with LAMP1, a marker for lysosomes.
Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder: Untargeted Metabolomics and Lipidomics Data Deposition
By onRaw data files for untargeted metabolomics and lipidomics were utilized in the study on brain lysosomes, identifying SLC45A1-related disease as a lysosomal disorder.
Measurement of Lysosomal pH Using LysoSensor Yellow/Blue DND-160
By onProtocol measures lysosomal pH with LysoSensor Yellow/Blue DND-160 dye. Steps include dye incubation, fluorescence reading, and pH calibration curve for accurate pH values.
Mass spectrometry analysis with Data Independent Acquisition (DIA)
By onDetailed protocol for DIA-based MS data acquisition method is provided here.
Co-Immunoprecipitation (co-IP) Protocol
By onCo-IP is a common method to study protein-protein interactions in cells.
Magic Red® Cathepsin B Assay Protocol
By onThe Magic Red Cathepsin B Assay Kit detects intracellular cathepsin B activity in live cells using a fluorescence-based assay with a red fluorescent signal. It utilizes a specific substrate that releases a fluorophore when cleaved by cathepsin B.
Rapid Isolation of Lysosomes (LysoIP) from mouse brain tissue
By onProtocol for rapid isolation of lysosomes from mouse brain tissue using LysoIP developed to assess lysosomal protein profiles. Enables detailed proteomic analysis of lysosomes, critical for biomolecule degradation and recycling.
Benzoyl-Hexose derivatization
By onHexose derivatization boosts hexose sugar detection in samples for LC-MS analysis.
ROS Measurement Using CellROX
By onProtocol uses CellROX fluorescent probe to measure cellular oxidative stress via flow cytometry.
Assessment of Mitochondrial OCR For SH-SY5Y Cell using Agilent Seahorse XFe Cell Mito Stress Test
By onProtocol assesses mitochondrial function in SH-SY5Y cells using Agilent Seahorse XFe Cell Mito Stress test measuring OCR. It also examines impact of iron supplementation and ROS inhibition on mitochondrial function.
Mouse Cortical Neuron isolation
By onProtocol for isolating and preparing cortical neurons from E16-17 mouse embryos is outlined in detail.
Flow Cytometry Protocol for LAMP1 Staining V.2
By onProtocol details staining of LAMP1 in human cells using flow cytometry.
PEI transfection protocol for imaging of transient expression of target protein
By onTransfect U2OS cells with PEI for transient expression of GFP-fused protein.
BioID: Identifying Protein-Protein Interactions in Living Cells
By onBioID method uses BirA* to biotinylate proteins near a target protein in cells. This allows isolation of biotinylated proteins for identification using mass spectrometry.
Protocol to generate stable cell line using HEK293 Flp-ln TREx cells
By onHEK293 FlpIn TREx cells were transfected to establish stable cell lines.
Sample preparation for proteomic analysis of LysoIP
By onSample preparation in proteomics is vital for accurate data acquisition and analysis using techniques like LC-MS. Methods range from in-gel digestion to in-solution prep, all following similar principles and steps.
Cloning with restriction enzymes protocol
By onLearn how to efficiently insert your gene fragment into a vector using restriction enzymes for quick cloning.
