Protocols for “LRRK2 mutations block NCOA4 trafficking upon iron overload leading to ferroptotic death”
By onLRRK2-G2019S affects iron homeostasis in macrophages by dysregulating iron-related proteins. This leads to impaired ferritinophagy, increased oxidative stress, and cell death, highlighting LRRK2's role in iron regulation in Parkinson's Disease.
An LRRK2 variant blocks NCOA4 trafficking upon iron overload, leading to ferroptotic death
By onWe examined the impact of mutant LRRK2-G2019S on iron homeostasis within a model macrophage cell line. Results revealed a dysregulation of iron-related proteins in steady state and a complete block in the degradation of the ferritinophagy NCOA4.
Protocols for “STING dampens the unfolded protein response to enable the presentation of self-antigens on MHC-I during inflammation”
By onSTING pathway regulates MitAP through the UPR, establishing it as a key immune regulator in autoimmunity and PD.
Dataset for “An LRRK2 variant blocks NCOA4 trafficking upon iron overload, leading to ferroptotic death”
By onLRRK2 as a key regulator of iron homeostasis and point to the need for an increased focus on the mechanisms of iron dysregulation in PD
Proteomics for “An LRRK2 variant blocks NCOA4 trafficking upon iron overload, leading to ferroptotic death”
By onLRRK2 as a key regulator of iron homeostasis and point to the need for an increased focus on the mechanisms of iron dysregulation in PD
ASAP Collaborative Research Network (CRN) Impact Report 2025-2026
As an extension of the ASAP CRN Impact Report 2020–2024, this report highlights the cross-cutting scientific themes, field-enabling resources, infrastructure efforts, and key discoveries accelerating breakthroughs in PD research across our Collaborative Research Network (CRN).
Rotarod with Photometry
By onThis protocol describes collecting in vivo fiber photometry data during rotarod behavior
Ex Vivo Mouse Brain Slice Whole-Cell Patch-Clamp Electrophysiology – Lerner Lab
By onThis protocol outlines steps for performing ex vivo slice whole-cell patch-clamp electrophysiology experiments, including optogenetic stimulation introduced through viral injection or transgenic expression.
Stereotaxic Surgery for Viral Injections or Brain Implant
By onThis protocol details stereotaxic surgery for injection of AAVs or implantation of fiber photometry probe(s) in mouse brain.
Transcardial Perfusion for Immunohistochemistry
By onThis protocol details transcardial perfusion of mice with PBS followed by 4% Paraformaldehyde
Thalamus orchestrates local acetylcholine-dependent dopamine release in the learning striatum
By onThalamic input can trigger dopamine release in the striatum during motor learning, likely reflecting action vigor rather than motor error. Changes in this mode of dopamine release may contribute to motor and non-motor symptoms of PD.
Dataset for Article “Thalamus orchestrates local acetylcholine-dependent dopamine release in the learning striatum”
By onThis is the primary dataset for the article by Miller-Hansen et al "Thalamus orchestrates local acetylcholine-dependent dopamine release in the learning striatum"
Code for Miller-Hansen article “Thalamus orchestrates local acetylcholine-dependent dopamine release in the learning striatum”
By onThis is the code generated by Miller-Hansen for analyzing output from Guppy.
Pavlovian protocol – Fushiki et al 2024
By onPavlovian Conditioning Protocols for Mouse Behavioral Studies Derived from Fushiki et al 2024.
Cryo-EM structure of soluble VPS13C suggests its regulation by a conformational switch and by calmodulin
By onStructural studies of the Parkinson's protein VPS13C provide a molecular basis for understanding its function and regulation.
Q-DOAS: A Proximity Quenching Assay for Real-Time Detection of Early Protein Aggregation Events
By onWe describe Q-DOAS, a plate-reader assay that quantifies protein self-assembly in real time via proximity-quenching of a single, site-specifically conjugated dye (BODIPY-TMR), and which allows quantification of pre-amyloid oligomers.
Datasets for Belal et al. ‘Cholinergic interneuron control of GABAergic circuits targeting spiny projection neurons is disrupted in parkinsonian models’
By onDeposit contains processed data spreadsheets and SVG figure panels generated from the raw electrophysiology, imaging and in-situ hybridization recordings.
Culturing, transfection and NBD-labeled lipid uptake assay in HeLa-CDC50A-OE cells
By onAssay for NBD-labeled lipid uptake in HeLa-CDC50A-OE cells includes seeding, transfection, and flow cytometry to measure flippase construct activity, enabling quantitative assessment of lipid uptake.
Flow cytometry data from the manuscript “Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mamma-lian Cells”
By onSummary: The manuscript presents a fluorescence-gated flow cytometry method to measure lipid flippase activity in mammalian cells.
Single-nucleus RNA (snRNA) sequencing
By onStep-by-step protocol describing the snRNA sequencing workflow for nuclei isolated from post-mortem human brain tissue.
