Rating scale for Parkinsonian motor signs in macaques and other non‐human primates
By Emma Sherrell onThis protocol details rating scale for Parkinsonian motor signs in macaques and other non‐human primates.
Comparison of alternative pre-mRNA splicing and gene expression patterns in midbrain lineage cells carrying familial Parkinson’s disease mutations
By Emma Sherrell onThe authors investigated the effects of individual familial PD mutations by developing a medium-throughput platform using genome editing to install individual PD mutations in human pluripotent stem cells (hPSCs) that the authors subsequently differentiated into midbrain lineage cells that include dopaminergic (DA) neurons. Analysis revealed that PD mutations increase pre-mRNA splicing changes.
PGK mScarlet-Synaptophysin
By Emma Sherrell onPlasmid: Synaptophysin, mScarlet (N terminal on backbone).
PGK EGFP-RAB3A-Q81L
By Emma Sherrell onPlasmid: RAB3A, mutation Q81L, EGFP (N terminal on backbone).
PGK EGFP-RAB3A-T86E
By Emma Sherrell onPlasmid: RAB3A, mutation T86E, EGFP (N terminal on backbone).
PGK EGFP-RAB3A-T86A
By Emma Sherrell onPlasmid: RAB3A, mutation T86A, EGFP (N terminal on backbone).
Microbiome-based biomarkers to guide personalized microbiome-based therapies for Parkinson’s disease
By Emma Sherrell onNot all persons with PD have a dysbiotic microbiome, and not all dysbiotic PD microbiomes have the same features. The authors have developed an intuitive and easily modifiable method to identify the optimal candidates for microbiome-based clinical trials, and subsequently, for treatments that are personalized for each individual's dysbiotic features.
R Code (RNA editing)
By Emma Sherrell onThe repository at https://github.com/karishdsa/ipscAstrNeurCocul contains single-cell and bulk RNA sequencing analysis of the cell culture models utilized in the study. This current repository contains the analysis of RNA editing in the cell culture models, as well as in a published dataset of post-mortem brain samples.
Alpha-synuclein aggregates trigger anti-viral immune pathways and RNA editing in human astrocytes
By Emma Sherrell onThis current repository contains single-cell and bulk RNA-sequencing analysis of the cell culture models utilized in the study. The repository at https://github.com/aaronwagen/Astrocytes_editing contains the analysis of RNA editing in the cell culture models, as well as in a published dataset of post-mortem brain samples.
Script for the spike numbers in LRRK2 KI mice
By Emma Sherrell onThe script determines the number of spikes for each current injected step in the manuscript titled R1441C and G2019S LRRK2 knockin mice have distinct striatal molecular, physiological, and behavioral alterations by Xenias et al. In addition, various parameters such as the threshold, maximum firing, rheobase, dy/dt, and FWHM are determined.
Fiber photometry data analysis
By Emma Sherrell onCustom code for fiber photometry data pre-processing (bleaching correction, DF/F calculation, behavioral parsing...) are provided.
Proportion and distribution of neurotransmitter-defined cell types in the ventral tegmental area and substantia nigra pars compacta
By Emma Sherrell onThe relative distributions and proportions of neurotransmitter-defined cell types across VTA and SNc have remained unclear. The data shown here complement recent single-cell RNAseq studies and support a more diverse landscape of neurotransmitter-defined cell types in VTA and SNc.
GLP-1 receptor agonism ameliorates Parkinson’s disease through 1 modulation of neuronal insulin signalling and glial suppression
By Emma Sherrell onNeuronal insulin resistance is linked to the pathogenesis of Parkinson's disease through unclear, but potentially targetable, mechanisms. The authors delineated neuronal and glial mechanisms of insulin resistance and glucagon-like 1 peptide (GLP-1) receptor agonism in human iPSC models of synucleinopathy.