all data related to “Genetically Encoded and Modular SubCellular Organelle Probes (GEM-SCOPe) reveal lysosomal and mitochondrial dysfunction driven by PRKN knockout”
By onRaw imaging data for "Genetically Encoded and Modular SubCellular Organelle Probes (GEM-SCOPe) reveal lysosomal and mitochondrial dysfunction driven by PRKN knockout"
Dynamic basal ganglia output signals license and suppress forelimb movements-Datasets
By onThis repository contains the datasets and analysis files associated with the study by Falasconi, Kanodia, and Arber (2025).
Immunostaining and Western blot images
By onRaw immunostaining and western blot images included in Adami et al, 2025.
Patch-clamp recordings of SNpc dopamine neurons from midbrain slices
By onProtocol for patch-clamp recordings of SNpc dopamine neurons from midbrain slices
Raw tomogram of GFP-a-synuclein inclusion in primary mouse neuron expressing GFP-a-synuclein, seeded with MSA aggregates
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
Raw tomogram of GFP-a-synuclein inclusion in primary mouse neuron expressing GFP-a-synuclein, seeded with PFFs
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
Raw tomogram of endogenous a-synuclein inclusion in primary mouse neurons seeded with PFFs
By onRaw data associated with DOI: 10.1038/s41467-021-22108-0
Quantification tables of SNr molecular organization
By onConfocal image quantification tables associated with the manuscript Segregated basal ganglia output pathways correspond to genetically divergent neuronal subclasses.
Raw images of SNr molecular anatomy
By onRaw images associated with the manuscript Segregated basal ganglia output pathways correspond to genetically divergent neuronal subclasses.
Data for Jain et al Adaptor Protein-3 Produces Synaptic Vesicles that Release Phasic Dopamine
By onPrimary data for Jain et al "Adaptor protein-3 produces synaptic vesicles that release phasic dopamine" (Proc Natl Acad Sci. 2023; 120(42):e2309843120. doi: 10.1073/pnas.2309843120)
Immunohistology for Mouse Brain Sections
By onThis protocol outlines the general steps for preparing mouse brain tissue for immunostaining and histology analysis in the Ding lab.
Immunohistochemistry (Brain organoids)
By onProtocol for preparing brain organoid slides and performing immunostainings to analyze protein expression and localization in the 3D model.
Microscopy-based bead assay
By onThis protocol describes the procedure to perform microscopy-based bead assay.
Microscopy-based pUb-coverage measurements of mitochondria in iNeurons
By onAssociated with preprint:
Live Imaging of Primary Mouse Neuron Cultures
By onThis protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging described involves labeling of dopamine neurons with a fluorescent DAT ligand and using virally encoded pHlourin sensors to measure vesicular release of neurotransmitter as the neurons are electrically stimulated. This technique was used in Jain et al., 2023 to compare vesicular release in neurons between various transgenic knockout mouse lines.
Evaluation of pUb kinetics using 3D-SIM
By onAssociated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1
Immunocytochemistry of cultured human Medium Spiny Neurons (MSNs)
By onThis protocol describes the immunolabelling of one or several protein targets on PFA-fixed cell culture on glass coverslips.
Live imaging and analysis to investigate phase separation properties of NEMO during mitophagy
By onProtocol for live imaging and analysis to investigate phase separation properties of NEMO during mitophagy.
Primary confocal microscopy data associated with the manuscript “Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B” (doi: 10.1101/2023.06.07.544051)
By onConfocal microscopy images, CellProfiler Excel files of Pearson's coefficients between TMEM55B/RILPL1 or pRab10/RILPL1 in R1441C MEF or VPS35 MEF cells, and Prism files of graphed Pearson's coefficients for each condition, as seen in doi: 10.1101/2023.06.07.544051.
