Postnatal astrocyte labeling by electroporation
By onHere, we provide a step-by-step guide to perform Postnatal Astrocyte Labeling by Electroporation (PALE), detailing the preparation, electroporation procedure, and post-electroporation analysis.
Immunocytochemistry – subcellular localization of ATP13A4
By onThis protocol describes an optimized method for visualizing the subcellular localization of over-expressed ATP13A4 in HeLa cells using immunocytochemistry.
IHC staining (DAB) of Iba1 and Gfap
By onThis protocol details the IHC staining (DAB) of Iba1 and Gfap.
Synapse staining – mouse brain sections
By onThis protocol details a method to visualize and quantify excitatory synapses using mouse brain sections.
Protocol to study synapse density or volume— SynDOVE—in brain using confocal microscopy and Imaris three-dimensional surface rendering software
By onGuide for analyzing for analysis of synaptic loci density and pre- and postsynaptic volumes (SynDOVE). from confocal images of pre- and postsynaptic loci in fixed brain sections with Imaris software.
quantification data related to “ATP13A2 Loss of Function-Driven Polyamine Dysregulation Induces SAM Depletion and Epigenetic Astrocyte Toxicity”
By onquantification data related to "ATP13A2 Loss of Function-Driven Polyamine Dysregulation Induces SAM Depletion and Epigenetic Astrocyte Toxicity"
Tabular data from Mitochondrial Proteostasis Cryo-ET Analysis
By onTabular output data from the Mitochondrial Proteostasis Cryo-ET Analysis as demonstrated in Ehses et al., 2026.
Fluorescence microscopy analysis of PINK1 localization upon GTPP treatment
By onPINK1-GFP Knock-in cells were stained with the mitochondrial membrane potential–dependent dye MitoSpy Red CMXRos and nuclear DNA dye Hoechst 33342 following treatment with 10µM G-TPP over multiple time points.
ADP-bound human mtHsp60:mtHsp10 single-ring complex (C7), SPA reconstruction
By onCryo-EM SPA density-map of a ADP-bound human mtHsp60:mtHsp10 single-ring complex, SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 double-ring complex (C7), SPA reconstruction
By onCryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 double-ring complex (C7), SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 single-ring complex (C7), SPA reconstruction
By onCryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 single-ring complex, SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 half-football complex (C7), SPA reconstruction
By onCryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 half-football complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 bullet complex (C7), SPA reconstruction
By onCryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 bullet complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 football complex (D7), SPA reconstruction
By onCryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 football complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
SPA dataset of in vitro human mHsp60 assemblies
By onDataset used for Single-particle cryo-EM analysis of recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP. Purified proteins were incubated under defined conditions and vitrified at multiple time points.
mtHsp60:mtHsp10 football complex, cryo-ET STA from HeLa
By onSubtomogram average of the mtHsp60:mtHsp10 football complex, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
In situ cryo-ET dataset of mitochondria in HeLa cells
By onDataset of tilt-series from in situ cry-electron tomography. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
mtHsp60 single- ring complex, cryo-ET STA from HeLa
By onSubtomogram average of the mtHsp60 single- ring complex, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
mtHsp60:mtHsp10 half-football complex, cryo-ET STA from HeLa
By onSubtomogram average of the mtHsp60:mtHsp10 half-football complex, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
55S mitochondrial ribosome, cryo-ET STA from HeLa
By onSubtomogram average of the 55S mitochondrial ribosome, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
