Standard Operating Procedure (SOP) for Combined ICA and Systemic Administration of MPTP: The Overlesioned (Bilateral Asymmetric) Non-human Primate Model
By onThis standard operating procedure (SOP) describes safe methods for the use of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates (adult macaque monkeys).
Generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line
By onThis protocol details the generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line.
Sleep-Wake Recording in the Turek Laboratory
By onSurgery and recording protocol for measuring sleep-wake behavior in mice for Turek Laboratory.
Sleep-Wake Recording in Dan lab
By onSurgery and Setups for Recording 24-Hour Natural Sleep/Wake Cycles in Mice.
Localised axotomy of human Cortical Neurons (CNs) from induced pluripotent stem cells (iPSCs)
By onThis protocol describes the process followed to perform localised axotomy of iPSC-derived human cortical axons cultured within fluid-walled dumbbells in 6 cm TCT-treated Petri dishes.
Viral-mediated short-hairpin RNA knockdown
By onStereotaxic injection of viral vectors for gene knockdown experiments: The AAV vectors for knocking down the gene of interest were custom-made with Vector Biolabs using AAV-GFP-U6 vector for AAV1 packaging (Cat no, 7040, Vector Biolabs).
Preparation and Administration of Systemic 4-AP
By onProcedure for preparation and systemic administration of 4-AP used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR knock-in validation
By onProcedure for CRISPR knock-in validation used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR knock-in (endogenous tagging)
By onProcedure for CRISPR knock-in (endogenous tagging) used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
CRISPR nuclease (CRISPRn) genome editing
By onProcedure for CRISPR nuclease (CRISPRn) genome editing used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Magnetic resonance imaging (MRI) of mouse brain
By onMagnetic resonance imaging (MRI) of mouse brain.
Quantification of p62-positive inclusions
By onQuantification of p62-positive inclusions in the rodent brain
