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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
mRNA purification by translating ribosome affinity purification (TRAP) of DAT-expressing cells (dopaminergic neurons) in mouse ventral midbrain
This protocol describes the capture of eGFP-L10a-tagged ribosomes and mRNA from DAT-expressing cells (dopaminergic neurons) in mouse ventral midbrain.
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Unconventional secretion of α-synuclein mediated by palmitoylated DNAJC5 oligomers
The secretion of endogenous α-syn mediated by DNAJC5 is also found in a human neuroblastoma cell line, SH-SY5Y, differentiated into neurons in the presence of retinoic acid, and in human-induced pluripotent stem cell-derived midbrain dopamine neurons. We propose that DNAJC5 forms a palmitoylated oligomer to accommodate and export α-syn.
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Impaired dopamine release in Parkinson’s disease
Evidence indicates that impaired dopamine release can result from disruption to a diverse range of Parkinson’s disease-associated genetic and molecular disturbances, and can be considered as a potential pathophysiological hallmark of Parkinson’s disease.
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Differentiation of human medium spiny neurons (MSNs) from induced pluripotent stem cells (iPSCs)
This protocol generates human medium spiny neurons (MSNs) from induced human pluripotent stem cells (iPSCs).
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Datasets and key resources used in Do, Quyen B. et al. (2023) – Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation
The results highlight the unique utility of modelling striatal neurons in a modular and highly physiological circuit which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.
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Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
This protocol described the production of human cortical neurons from induced pluripotent stem cells in cocultured with commercially available rat astrocytes.
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Single-cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease
Published: The authors used two approaches to ask which genes are expressed during aging in dopaminergic neurons; the cell type affected in Parkinson’s disease. View original preprint.
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SpatialBrain.org – a platform to view integrated data from Wade-Martins Laboratory of Molecular Neurodegeneration
A platform to view integrated data from the Wade-Martins Laboratory of Molecular Neurodegeneration.
Results and data from Kilfeather, Khoo, et al. 2023 (Manuscript in review):
Spatial Transcriptomic Analyses:
-Cell Type Markers
-SN/VTA Markers in Dopaminergic Neurons
-Cell Number Changes in Age
TRAP Analyses:
-Dopaminergic Markers
-Dopaminergic Aging
-Alternative Splicing in Dopaminergic Neurons
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Inhibition of striatal dopamine release by the L-type calcium channel inhibitor isradipine co-varies with risk factors for Parkinson’s
Published: This data show that LTCC function in DA axons, and isradipine effect, are locally governed and suggest they vary in a manner that in turn might impact on, or reflect, the cellular stress that leads to parkinsonian degeneration. View original preprint.
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Quantification of extracellular 𝜶-synuclein assay
This protocol uses a classical ELISA experimental design with sensitive electrochemical detection to provide a measure of 𝜶-synuclein concentration in the conditioned media of cultured iPSC-derived human cells.
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