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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Differentiation of SH-SY5Y cells
This protocol describes a standard procedure to differentiate SH-SY5Y cells into dopaminergic neurons using retinoic acid.
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Protocol
Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
This protocol described the production of human cortical neurons from induced pluripotent stem cells in cocultured with commercially available rat astrocytes.
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Article
Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation
The results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.
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Article
Single cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease
These datasets (accessible at spatialbrain.org) represent an invaluable resource for the investigation into spatially governed gene expression in brain cells in health, aging, and disease.
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Dataset
Welcome to SpatialBrain
A platform to view integrated data from the Wade-Martins Laboratory of Molecular Neurodegeneration.
Results and data from Kilfeather, Khoo, et al. 2023 (Manuscript in review):
Spatial Transcriptomic Analyses:
Cell Type Markers
SN/VTA Markers in Dopaminergic Neurons
Cell Number Changes in Age
TRAP Analyses:
Dopaminergic Markers
Dopaminergic Aging
Alternative Splicing in Dopaminergic Neurons
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Article
Inhibition of striatal dopamine release by the L-type calcium channel inhibitor isradipine co-varies with risk factors for Parkinson’s
Published: This data show that LTCC function in DA axons, and isradipine effect, are locally governed and suggest they vary in a manner that in turn might impact on, or reflect, the cellular stress that leads to parkinsonian degeneration. View original preprint.
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Article
Sst+ GPi output neurons provide direct feedback to key nodes of the basal ganglia and drive behavioral flexibility
Preprint: The GPi-LHb pathway is the main output of the basal ganglia suggested to shape motivated behaviors. Here, the authors propose that GPi Sst+ neurons drive behavioral flexibility through a direct feedback signal to balance the activity of key nodes in the basal ganglia.
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Lab Resource
pCMV-pOTC-3H-GFP
Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle.
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