This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Unconventional secretion of alpha-synucein mediated by palmitoylated DNAJC5 oligomers
This protocol, carried out according to the manufacturer’s instructions, uses a classical ELISA experimental design with sensitive electrochemical detection to provide a measure of 𝜶-synuclein concentration in the conditioned media of cultured iPSC-derived human cells.
Teams
Teams
Protocol
Differentiation of human medium spiny neurons (MSNs) from induced pluripotent stem cells (iPSCs)
This protocol generates human medium spiny neurons (MSNs) from induced human pluripotent stem cells (iPSCs).
Teams
Dataset
Datasets and key resources used in Do, Quyen B. et al. (2023) – Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation
The results highlight the unique utility of modelling striatal neurons in a modular and highly physiological circuit which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.
Teams
Protocol
Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
This protocol described the production of human cortical neurons from induced pluripotent stem cells in cocultured with commercially available rat astrocytes.
Teams
Article
Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation
Published: The results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients. View original preprint.
Teams
Article
Single-cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease
Published: The authors used two approaches to ask which genes are expressed during aging in dopaminergic neurons; the cell type affected in Parkinson’s disease. View original preprint.
Teams
Dataset
Welcome to SpatialBrain
A platform to view integrated data from the Wade-Martins Laboratory of Molecular Neurodegeneration.
Results and data from Kilfeather, Khoo, et al. 2023 (Manuscript in review):
Spatial Transcriptomic Analyses:
Cell Type Markers
SN/VTA Markers in Dopaminergic Neurons
Cell Number Changes in Age
TRAP Analyses:
Dopaminergic Markers
Dopaminergic Aging
Alternative Splicing in Dopaminergic Neurons
Teams
Article
Inhibition of striatal dopamine release by the L-type calcium channel inhibitor isradipine co-varies with risk factors for Parkinson’s
Published: This data show that LTCC function in DA axons, and isradipine effect, are locally governed and suggest they vary in a manner that in turn might impact on, or reflect, the cellular stress that leads to parkinsonian degeneration. View original preprint.
Teams
Teams
Teams
Teams
Teams
Article
Sst+ GPi output neurons provide direct feedback to key nodes of the basal ganglia and drive behavioral flexibility
Preprint: The GPi-LHb pathway is the main output of the basal ganglia suggested to shape motivated behaviors. Here, the authors propose that GPi Sst+ neurons drive behavioral flexibility through a direct feedback signal to balance the activity of key nodes in the basal ganglia.
Teams