Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Protocol

Protocol for hippocampal neuronal cultures

This protocol details the procedure for preparation of neuronal cultures from mice hippocampi as it was performed in https://doi.org/10.1083/jcb.202010004 but can also be used to prepare cultures of cortical neurons.

Protocol

Optogenetic experiments with iLID system

This protocol details experiments with the iLID optogenetic system as performed to acutely recruit Miro to mitochondria in https://doi.org/10.1083/jcb.202010004.

Protocol

Protocol for Image processing and analysis of VPS13D recruitment to mitochondria

This protocol details the image processing and analysis of VPS13D recruitment to mitochondria as it was performed in https://doi.org/10.1083/jcb.202010004. The first part details the analysis of acute optogenetic recruitment, and the second part of basal recruitment under different conditions.

Protocol

Hippocampal Neuronal Culture

This protocol describes the procedure for hippocampal neuronal cultures from new – born mouse pups.

Article

A possible role for VPS13-family proteins in bulk lipid transfer, membrane expansion, and organelle biogenesis.

This review focuses on the structure and function of the VPS13 family of proteins and discusses the prevailing hypthoses in the field regarding its role in lipid transport.

Article

Insights into VPS13 properties and function reveal a new mechanism of eukaryotic lipid transport

This review focuses on the disssecting the VPS13 family of proteins and their novel role in mediating lipid transfer between organelles.

Article

Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9

ATG9 is the only core autophagy transmembrane protein present at nerve terminals and links synaptic activity with autophagy, but little is known about its traffic. The authors found that ATG9 is a component of vesicles that undergo exo-endocytosis at synapses and that synaptojanin 1 mutations disrupt ATG9 activity at synapses. View preprint.

Article

VPS13D bridges the ER to mitochondria and peroxisomes via Miro

VPS13C mutations are implicated in PD. The authors found that VPS13D, a closely related protein, can mediate a bridge between the peroxisome and different organelles (ER or mitochondria). Further, they identified the specific ER and mitochondrial proteins that facilitated this interaction resulting in a dysregulation of lipid flux between them. View preprint.

Protocol

VPS13D DNA plasmid generation

This protocol describes the basic molecular cloning technique utilized for the generation of VPS13D constructs in VPS13D bridges the ER to mitochondria and peroxisomes via Miro. This protocol and the enzymes included in it are commercialized by Takara Bio.