Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Protocol

iPSC differentiation into Macrophages

This protocol describes iPSC differentiation into macrophages.

Protocol

Cell culture on EM grids and fluorescence microscopy imaging

This protocol describes the general procedure of seeding mammalian cells on EM grids and confocal fluorescence microscopy imaging of grids for subsequent cryo-tomography experiments, performed.

Protocol

A fluorescence-based in vitro scrambling assay for yeast MCP1 and human XK

Detailed procedure of purification, reconstitution, and scrambling assay for both MCP1 and XK.

Article

Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology

Published: Mutations in synaptojanin 1 (SJ1) and auxilin have been implicated in PD. The authors show that auxilin knockout mice have similar nigrostriatal changes to SJI mutant mice. Furthermore, auxilin/SJ1 double mutant mice have more severe defects and shower lifespans, showing the mutations have a synergistic interaction. View original preprint.

Article

Structural and biochemical insights into lipid transport by VPS13 proteins

Published: VPS13 proteins form conduits between organelles at contact sites that allow for bulk lipid flow between them. The authors present a crystal structure of how the adaptor binding domain of VPS13 interacts with Pro-X-Pro motifs present in VPS13 receptors at organellar membranes. View original preprint.

Article

SHIP164 is a chorein motif lipid transfer protein that controls endosome–Golgi membrane traffic

Published: The authors show that SHIP164 shares strong structural similarities with VPS13 and ATG2 and, like these two proteins, functions as a lipid transport protein. SHIP164 localizes to clusters of endocytic vesicles and loss of SHIP164 disrupts retrograde traffic of certain organelles to the Golgi complex. View original preprint.

Article

ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling

Published: Mutations in VPS13C cause early onset, autosomal recessive PD. VPS13C encodes a lipid transfer protein that is localized to ER-endosome/lysosome contact sites. The authors demonstrate that depletion of VPS13C causes an accumulation of lysosomes and activated STING, suggesting a link between ER-lysosome lipid transfer and innate immune activation. View original preprint.

Article

In situ architecture of the lipid transport protein VPS13C at ER-lysosomes membrane contacts

Publication: Loss-of-function mutations in VPS13C are responsible for rare cases of familial early onset Parkinson’s disease. Using cryo-ET, the authors provide in-situ evidence for a bridge-model of VPS13 in lipid transport. View the original preprint.

Protocol

Optogenetic experiments with iLID system

This protocol details experiments with the iLID optogenetic system as performed to acutely recruit Miro to mitochondria in https://doi.org/10.1083/jcb.202010004.

Protocol

Protocol for Image processing and analysis of VPS13D recruitment to mitochondria

This protocol details the image processing and analysis of VPS13D recruitment to mitochondria as it was performed in https://doi.org/10.1083/jcb.202010004. The first part details the analysis of acute optogenetic recruitment, and the second part of basal recruitment under different conditions.

Protocol

Hippocampal Neuronal Culture

This protocol describes the procedure for hippocampal neuronal cultures from new – born mouse pups.

Article

A possible role for VPS13-family proteins in bulk lipid transfer, membrane expansion, and organelle biogenesis.

Review: This review focuses on the structure and function of the VPS13 family of proteins and discusses the prevailing hypthoses in the field regarding its role in lipid transport.

Article

Insights into VPS13 properties and function reveal a new mechanism of eukaryotic lipid transport

Review: This review focuses on the disssecting the VPS13 family of proteins and their novel role in mediating lipid transfer between organelles.

Aligning Science Across Parkinson's
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