Generation of stable ATG3-mCherry wild-type or mutans HeLa cells with HaloTag-LC3B using lentivirus
By savannah onThis protocol details the generation of stable ATG3-mCherry wild-type or mutant HeLa cells with HaloTag-LC3B using lentivirus.
Generation of ATG3 KO Hela cells stably expressing HaloTag-LC3B1`
By savannah onThis protocol details generation of ATG3 KO Hela cells stably expressing HaloTag-LC3B.
Synapsin E-domain is essential for α-synuclein function
By savannah onTogether with previous studies implicating the E-domain in clustering SVs, the authors’ experiments advocate a cooperative role for these two proteins in maintaining physiologic SV clusters. View original preprint.
PINK1: From Parkinson’s disease to mitophagy and back again
By savannah onThis perspective discusses the implications of a 2010 PLOS Biology paper that shed light on the functional importance of PINK1 in the mitophagy cascade. https://doi.org/10.1371/journal.pbio.1000298
SpatialBrain.org – a platform to view integrated data from Wade-Martins Laboratory of Molecular Neurodegeneration
By savannah onA platform to view integrated data from the Wade-Martins Laboratory of Molecular Neurodegeneration. Results and data from Kilfeather, Khoo, et al. 2023 (Manuscript in review): Spatial Transcriptomic Analyses: -Cell Type Markers -SN/VTA Markers in Dopaminergic Neurons -Cell Number Changes in Age TRAP Analyses: -Dopaminergic Markers -Dopaminergic Aging -Alternative Splicing in Dopaminergic Neurons
ATG3 construct cloning
By savannah onCloning of ATG3 construct for production of recombinant (GFP tagged) ATG3 variants.
LC3 lipidation assay for ATG3 mutants
By savannah onLC3 lipidation reaction assay on liposomes (SUVs, All Unilamellar vesicles).
GFP-ATG3 GUV Assay
By savannah onLC3 lipidation reaction assay on Giant Unilamellar vesicles (GUVs).
Whole proteome copy number dataset in primary mouse cortical neurons
By savannah onIn this article, the authors provide a proteomic reference dataset that has been generated to identify proteins and quantify their level of expression in primary mouse cortical neurons. It represents a summary analysis of previously published data in (Antico et al., 2021).
Primary data associated with the manuscript “Whole proteome copy number dataset in primary mouse cortical neurons” (doi: 10.1016/j.dib.2023.109336)
By savannah onRaw data reported in Supplementary table in "Whole Proteome Copy Number Dataset in Primary Mouse Cortical Neurons."
Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice
By savannah onTaken together, the authors’ findings reveal the striking resilience of DA-dependent motor functions in the context of a near-abolition of phasic DA release, shedding new light on why extensive loss of DA innervation is required to reveal motor dysfunctions in PD.
SINTBAD-GFP: Expression and purification
By savannah onThis protocol describes how to express and purify human SINTBAD tagged C-terminally with eGFP.
NAP1-mCherry
By savannah onThis protocol describes how to express and purify human NAP1 tagged C-terminally with mCherry.
In vitro kinase assay: Phosphorylation of PI3Kc1 by TBK1and ULK1
By savannah onThis protocol describes in vitro kinase assay, in which PI3Kc is tested for phosphorylation by TBK1 and ULK1 complex either by western blotting (S29 on ATG14L subunit) or mass spectrometry analysis.
Co-immunoprecipitation protocol to study LRRK2 binding to Rab12 in a cell-based assay
By savannah onThe immunoprecipitation (IP) of FLAG-tagged LRRK2 from whole cell lysates to assess its interaction with Rab12. This method can be used to screen the impact that LRRK2 mutations have on its binding to Rab12 in cells, as well as the effect of any compound or cell treatment on LRRK2 interaction with Rab12.
Protocols for “Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice”
By savannah onThis is a collection of protocols for the manuscript "Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice."
PGK EGFP-LC3B
By savannah onMammalian expression of fluorescently-tagged marker for autophagic vesicles.
PGK 4xMito-mEmerald
By savannah onMammalian expression of fluorescently-tagged marker for mitochondria.
