FISH CODETECTION
By onDIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
Determining the effects of mecamylamine in the mouse striatum using a Conditioned Preference Place (CPP) paradigm
By onProtocol tests mecamylamine effects in mouse striatum using CPP paradigm to measure preference for drug-associated environment.
Intracranial injections of viral vectors in mouse midbrain and striatum
By onProtocol for injecting viral vectors in mouse midbrain and striatum is provided.
In vivo voltammetry and fiber photometry in the mouse striatum
By onProtocol measures dopamine and calcium levels in striatal dopaminergic axons in vivo via voltammetry and fiber photometry in response to electrical stimulation.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices following electrical or optogenetic stimulations
By onThis protocol is to assess changes in extracellular dopamine concentration following electrical or optogenetic stimulations in ex vivo mouse brain slices.
s-µIP Fabrication Process
By onThis protocol describes the fabrication of s-µIP microelectrodes. These devices are made of either one or two parylene-coated tungsten-carbon fiber assemblies, threaded through a silica tube.
Aspiration of Fluid in Semi-Sealed Chamber
By onProtocol for post-op maintenance of electrode chambers emphasizes fluid aspiration and contamination prevention to reduce infection risk. Regular cleaning is crucial for asepsis and preventing complications like meningitis.
Combined RNAscope and immunohistochemistry labeling of fresh mouse midbrain tissue
By onHere we describe labeling coronal sections of mouse substantia nigra (SNc) and ventral tegmental area (VTA) for the gene Slc17a6 (VGLUT2) and virally-expressed histone-tagged GFP.
Image Processing and 3D Reconstruction – Chen et al. 2025 NSMB
By onImage processing and 3D reconstruction applied in Chen et al. 2025 NSMB
Protocols for Goldman et al. 2025
By onAll protocols related to GEM-SCOPe and described in Glodman et al. 2025
Phosphatome-wide siRNA screen in 3T3 cells
By onProtocol details a phosphatome-wide siRNA screen in 3T3 cells to identify phosphatases counteracting LRRK2 for pRab12 and pRab10.
Lysosomal GCase (glucocerebrosidase) activity assay
By onMethod to measure lysosomal GBA1 enzyme activity using 4-MUG substrate at low pH to minimize non-lysosomal GCase interference. GBA1 activity higher in purified lysosomes than whole cell extract, confirmed by CBE inhibitor abolishing 4-MUG hydrolysis.
Preparation of Acute Brain Slices
By onThis protocol describes the steps for preparing acute brain slices.
Electrophysiology and 2-photon imaging of Ca+2-transients
By onProtocol for imaging dendritic calcium transients using 2-photon microscopy with a Ti:Sapphire laser. Fluorescence collected through objective, dichroic mirror, and filters, then detected by a GaAsP PMT. Output digitized for analysis.
Ex Vivo Electrophysiology – Ford Lab
By onThis protocol describes the steps to perform whole-cell electrophysiology recordings in acute brain slices.
Motor Behavioral Assessment
By onProtocol outlines motor behavior tests for Parkinson's disease in mice using open field, rotarod, cylinder, and balance beam tests. Mice were habituated for 30-40 min before tests conducted 3-4 weeks post-injections.
Mouse Whole Gut Transit Time
By onProtocol used in the Gradinaru lab for obtaining whole gut transit time for mice
