Astrocyte generation
By onA protocol for generating astrocytes from cortical neural progenitor cells using a commercial astrocyte media (AM) is described.
Primary Neuron Extraction
By onProtocol used in the Gradinaru lab to extract primary neurons from mouse on day E17
Transfection of HEK cells with Lipofectamine 3000
By onProtocol used in the Gradinaru lab to transfect HEK cells with lipofectamine 3000
Stimulation of DA neurons and microglia
By onThis protocol details how to stimulate microglia with IFN-gamma, poly-IC and TNF-alpha.
Transient Transfection of Cultured Mammalian Cells for Live 3D Fluorescence Microscopy
By onTransient Transfection of Cultured Mammalian Cells for Live 3D Fluorescence Microscopy
OA treatment and Quantification of Parkin recruitment to mitochondria
By onOA treatment and Quantification of Parkin recruitment to mitochondria
Isolation of pure neuromelanin extracts from cultured TR5TY6 neuroblastoma cells
By onIsolation of pure neuromelanin extracts from cultured TR5TY6 neuroblastoma cells
Formation of ventral midbrain Assembloid/Organoids
By onThis pilot establishes brain organoids from iPSC-derived vmDA neurons, optimizing growth conditions and exploring tri-culture integration with microglia to model neurodevelopment and cell-type interactions.
Ventral midbrain dopaminergic (mDA) Neuron Differentiation Protocol
By onOptimized protocol for generating authentic ventral midbrain dopaminergic neurons from human iPSCs enhances cell therapy and modeling, improves consistency across lines, and supports integration with organoid and assembloid systems.
Postnatal primary hippocampal neuronal cultures
By onThis protocol describes the preparation of postnatal primary hippocampal neuronal cultures.
Adherent mammalian Cell Culture maintenance
By onSubculturing involves transferring cells into fresh growth medium to maintain healthy cell cultures. It is a crucial step in the propagation of HEK293T, SH-SY5Y, and U2OS cell lines.
Mouse Cortical Neuron isolation
By onProtocol for isolating and preparing cortical neurons from E16-17 mouse embryos is outlined in detail.
Protocol to generate stable cell line using HEK293 Flp-ln TREx cells
By onHEK293 FlpIn TREx cells were transfected to establish stable cell lines.
