Cell culture, transfection, immunocytochemistry, and imaging (iPSC, iNeuorn, DA neuron, RPE cells)
By onThis protocol describes the maintenance, transfection, immunocytochemistry, and imaging of RPE1 and also transfection, immunocytochemistry, and imaging of iPSCs, i3 Neurons and DA neurons.
Generation of knockout iPSCs using CRISPR-Cas9 genome editing
By onThis protocol describes the genetic modification of induced pluripotent cells (iPSCs) using CRISPR-Cas9, including synthesis of gRNA plasmids, transfection, selection of clones, and sequencing of genomic DNA to confirm knockout generation. The steps
Primary hippocampal and cortical neuronal culture and transfection
By onThis protocol presents an established method to isolate and culture hippocampal and cortical neurons from rat and mouse brains along with a method to sparsely transfect these neurons.
Astrocyte generation
By onA protocol for generating astrocytes from cortical neural progenitor cells using a commercial astrocyte media (AM) is described.
