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  • LRRK2 R1441C/PINK1 Double mutant mouse embryonic fibroblasts

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    LRRK2 R1441C/PINK1 double mutant mouse embryonic fibroblasts were studied alongside homozygous mutant and littermatched wild-type cells.

  • H9 ES AAVS1-NGN2 FLAG-EEA1

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    ES cells were modified to create iNeurons expressing EEA1 with a 3xFLAG tag. NEUROG2 was introduced at the AAVS1 locus using CRISPR/Cas9. The cells are derived from human embryonic stem cells at the blastocyst stage.

  • H9 ES AAVS1-NGN2 FAM134C/A-/-

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    ES cells modified using CRISPR/Cas9 to lack FAM134C & A ER-phagy receptor. NEUROG2 construct introduced in AAVS1 locus. Derived from human embryonic stem cells at blastocyst stage.

  • HeLa HFT/TO PARK2 FBXO7−/−

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    HeLa cells with FBXO7/PARK15 deletion made by CRISPR/Cas9.

  • H9 ES AAVS-NGN2; FBXO7-/-

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    H9 ES cells modified with AAVS1-NGN2 and CRISPR-deleted FBXO7.

  • HEK293 TMEM115-3xHA GolgiTAG (CVCL_C8A6)

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    Cell line generated from HEK293 cells (Cat# CRL-1573, RRID:CVCL_0045) virally transfected with a construct expressing TMEM115-3xHA (GolgiTAG)

  • HeLa S3 penta KO-ATG13 KO

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    Cell Line: ID HeLa S3 penta KO-ATG13 KO cell line.

  • H9 ES AAVS1-NGN2 CCPG1-/-; PiggyBac-Keima-RAMP4

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    ES cells were modified using CRISPR/Cas9 to lack the ER-phagy receptor CCPG1 and express Keima-RAMP4 reporter. NEUROG2 construct was introduced in the AAVS1 safe harbor locus. The cells are embryonic stem cells derived from a female blastocyst.

  • H9 ES AAVS1-NGN2 FAM134C-/-; PiggyBac-Keima-REEP5

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    ES cells modified using CRISPR/Cas9 to lack FAM134C and express Keima-REEP5 ER-phagy reporter. NEUROG2 construct added in AAVS1 locus. Cell type: embryonic stem cell from human female at blastocyst stage.

  • H9 ES AAVS1-NGN2 FAM134A-/-; PiggyBac-Keima-REEP5

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    Cell Line: ES cells for making iNeurons lacking the ER-phagy receptor FAM134A and expressing the indicated Keima-REEP5 ER-phagy flux reporter.

  • SNCA E46K iPSCs

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    Available at Jax: SNV/WT, Rev/WT, and SNV/SNV of the E46K SNCA variant. Associated with Parkinson's disease with dementia. Parental line is KOLF2.1J (JIPSC1000).

  • KOLF2.1 iPSCs AAVS1-NGN2 ATG12-/-

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    Summary: iPSCs lacking autophagy factor ATG12 used to generate iNeurons from KOLF2.1 iPSCs with NEUROG2 introduced using CRISPR/Cas9 in AAVS1 safe harbor locus.

  • H9 ES AAVS1-NGN2 GRN-/-

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    H9 ES cells modified using CRISPR/Cas9 lack GRN gene and express NEUROG2 in AAVS1 safe harbor locus. Derived from human blastocyst, these embryonic stem cells can differentiate into iNeurons.

  • HeLa S3 ATG3 KO

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    HeLa cell line bearing knock out of ATG3.

  • HEK293 ::TMEM192-HA YIPF4-/-; YIPF3-/-

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    HEK293 ::TMEM192-HA YIPF4-/-; YIPF3-/- YIPF3/YIPF4-/- DKO HEK293; HEK293 YIPF3-/-;YIPF4-/- PubMed=37757899; Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Knockout cell: Method=CRISPR/Cas9; HGNC; 21023; YIPF3. Knockout cell: Method=CRISPR/Cas9; HGNC; 28145; YIPF4. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_D1J3 ! HEK293::TMEM192-3xHA YIPF4-/- Female Fetus Transformed cell line

  • HEK293::TMEM192-3xHA YIPF4-/-

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    HEK293 cell line with TMEM192 gene C-terminally tagged with 3xHA epitope using CRISPR/Cas9. YIPF4 knockout also done. Derived from fetal kidney, human origin, adenovirus transformed.

  • HeLa ::TMEM192-HA CALCOCO1-/-

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    HeLa ::TMEM192-HA CALCOCO1-/- PubMed=37757899; Population: African American. Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Knockout cell: Method=CRISPR/Cas9; HGNC; 29306; CALCOCO1. Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_D1KR ! HeLa::TMEM192-3xHA Female 30Y6M Cancer cell line

  • H9 ES AAVS1-NGN2 FAM134C/A-/-; PiggyBac-Keima-RAMP4

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    ES cells were modified using CRISPR/Cas9 to express a Keima-RAMP4 ER-phagy flux reporter and lack the FAM134C ER-phagy receptor. The cells were derived from a female human blastocyst stage embryonic stem cell line.

  • HeLa ::TMEM192-HA YIPF3-/-

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    HeLa ::TMEM192-HA YIPF3-/- PubMed=37757899; Population: African American. Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line). Knockout cell: Method=CRISPR/Cas9; HGNC; 21023; YIPF3. Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma NCBI_TaxID=9606; ! Homo sapiens (Human) RRID:CVCL_D1KR ! HeLa::TMEM192-3xHA Female 30Y6M Cancer cell line

  • H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; PiggyBac-Keima-REEP5

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    ES cells modified to produce iNeurons w/o ER-phagy receptor FAM134C/A/B, TEX264, and with Keima-REEP5 reporter. CRISPR/Cas9 used to introduce NEUROG2 construct in AAVS1 locus. Knockouts RETREG1/2/3, TEX264. Transfected with NEUROG2, REEP5, mKeima.

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