Protocol for the production of RAB1A-10xHis in HEK293Gnti cells
By onProtocol for production of RAB1A-10xHis
EXPRESSION AND PURIFICATION OF HUMAN NEMO (GST-GFP-NEMO)
By onThis protocol describes how to express and purify human NEMO (IKK-γ) tagged N-terminally with GST and EGFP.
Immunohistochemistry
By onThis protocol describes immunohistochemical staining of fixed brain sections.
Rab8a expression and purification
By onRecombinant Rab8a expression and purification protocol as used by the Leschziner and Reck-Peterson Labs
qPCR of α-synuclein, TNF and NF-κβ
By onA procedure for quantitative real time reverse transcription PCR of α-synuclein, TNF, and NF-κβ.
WIPI2d Expression and purification
By onProtocol for WIPI2d expression and purification from HEK cells.
Western blotting to detect ATP13A2 and ATP13A3
By onProtocol to detect ATP13A2 and ATP13A3 via Western Blotting.
LRRK2 RCKW Protein Purification
By onProtein purification protocol for tag-less LRRK2RCKW as done by Leschziner and Reck-Peterson Labs. Same protocol can be used to purify LRRK1RCKW as well. Original Protocol by David Snead. Modified by Yu Xuan Lin and Mariusz Matyszewski for publication.
Expression and purification for cryo-EM samples of FIP200NTD:ATG13(363-517)-ULK1(MIT) complexes
By onProtocol describing expression and purification protocols for cryo-EM samples of FIP200NTD:ATG13(363-517)-ULK1(MIT) complexes.
In vitro synthesis of PE2 (nCas9-MMLV RT fusion) polyA mRNA using T7 RNA polymerase
By onWe describe the preparation of synthetic capped and polyadenylated messenger RNA (mRNA) encoding a Cas9 nickase-reverse transcriptase fusion protein (PE2) for use in genome editing with a method called prime editing.
LRRK1 expression and purification
By onProtein purification protocol for full-length LRRK1 as done by Leschziner and Reck-Peterson Labs. Original protocol by Janice M. Reimer and Yu Xuan Lin.
Expression and purification MBP-ATG9 Constructs
By onExpression and purification from HEK cells of ATG13, ATG101 and FOLDON-ATG9A proteins.
Expression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs
By onExpression and purification Twin-STREP-FLAG tagged ATG13:ATG101 constructs
Purification of the PE2 nCas9-RT protein
By onThis protocol describes the process of expressing and purifying the nicking Cas9-MMLV RT fusion protein for prime editing.
Expression and purification of mCherry-OPTN
By onThis protocol describes the purification of mCherry-OPTN.
GFP-TBK1: expression and purification
By onThis protocol describes how to express and purify human TBK1 tagged N-terminally with eGFP.
Unilateral intranigral AAV alpha synuclein mouse model
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Expression and purification protocol of ULK1 Complex wt or K46I mutant
By onThis Protocol describes the expression and purification of ULK1 Complex wt or K46I mutant.
Purification of recombinant TauRD from E. coli
By onThe protocol outlines procedures for studying Tau protein aggregation in vitro using ThT fluorescence measurements.
