Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Article

Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore: Molecular dynamics simulation dat

Atomistic molecular dynamics simulation data set accompanying manuscript “Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore.”

Article

Peripheral MC1R activation modulates immune responses and is neuroprotective in a mouse model of Parkinson’s disease

Preprint: The present study investigates the impact of NDP-MSH, a synthetic melanocortin receptor (MCR) agonist that does not cross BBB, on the immune system and the nigrostriatal dopaminergic system in mouse model of PD.

Article

LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

The Leucine-rich repeat kinase-2 (LRRK2) G2019S mutation is one of the well-recognized genetic risk factors in Parkinson’s disease (PD). Increased LRRK2 activity was also observed in immune cells from PD patients. The authors generated and characterized a new T cell receptor (TCR) transgenic mouse strain bearing LRRK2 G2019S knock-in mutation.

Protocol

Striatal dopamine measurement through HPLC

Protocol for striatal dopamine measurement in mouse brain

Article

DOPA pheomelanin is increased in nigral neuromelanin of Parkinson’s disease

Published: Neuromelanin in the substantia nigra may be a key factor contributing to dopaminergic neuron vulnerability in Parkinson’s disease. Neuromelanin consists of pheomelanin and eumelanin moieties. Here, authors investigated the relative composition and specific roles of pheomelanin and eumelanin moieties of NM in PD. View original preprint.

Protocol

Post mortem human substantial nigra TH staining

This protocol is standardized for postmortem (frozen) SN tissue from pathologically diagnosed PD patients and control individuals for Immunofluorescence staining.

Article

Melanocortin 1 receptor activation protects against alpha-synuclein pathologies in models of Parkinson’s disease

Publication: The authors previously showed that MC1R signaling can facilitate nigrostriatal dopaminergic neuron survival. The present study investigates the neuroprotective potential of MC1R against neurotoxicity induced by alpha-synuclein, a key player in PD genetics and pathogenesis. View original preprint.

Protocol

Cryosectioning mouse brain

This protocol details the cryosectioning of the mouse brain.

 

Dataset

Structure of human ULK1 complex core (2:2:2 stoichiometry) of the ATG13(450-517) mutant

Organism(s): Homo sapiens
Expression System: Homo sapiens
Mutation(s): No
Deposited: 2023-05-05
Released: 2023-06-21
Deposition Author(s): Chen, M., Hurley, J.H.
Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Michael J. Fox Foundation

Dataset

Structure of human ULK1 complex core (2:2:2 stoichiometry) in the PI3KC3-C1 mixture

Organism(s): Homo sapiens
Expression System: Homo sapiens
Mutation(s): No
Deposited: 2023-05-04
Released: 2023-06-21
Deposition Author(s): Chen, M., Hurley, J.H.
Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Michael J. Fox Foundation

Dataset

Structure of human ULK1C:PI3KC3-C1 supercomplex

Organism(s): Homo sapiens
Expression System: Homo sapiens
Mutation(s): No
Deposited: 2023-05-05
Released: 2023-06-21
Deposition Author(s): Chen, M., Hurley, J.H.
Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Michael J. Fox Foundation

Dataset

Structure of human PI3KC3-C1 complex

Organism(s): Homo sapiens
Expression System: Homo sapiens
Mutation(s): No
Deposited: 2023-04-29
Released: 2023-06-21
Deposition Author(s): Chen, M., Hurley, J.H.
Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Michael J. Fox Foundation

Dataset

Structure of human ULK1 complex core (2:1:1 stoichiometry)

Organism(s): Homo sapiens
Expression System: Homo sapiens
Mutation(s): No
Deposited: 2023-04-28
Released: 2023-06-21
Deposition Author(s): Chen, M., Hurley, J.H.
Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Michael J. Fox Foundation

Protocol

Unilateral intranigral AAV alpha synuclein mouse model

This protocol details methods for the unilateral intranigral AAV alpha-synuclein injection.